Anti-Nanog antibody (Biotin) (ab84231)

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Key features and details

  • Biotin Rabbit polyclonal to Nanog
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Anti-Nanog antibody (Biotin)
    See all Nanog primary antibodies

  • Description

    Rabbit polyclonal to Nanog (Biotin)

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Specificity

    ab84231 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Nanog in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Highly pure (>98%) recombinant human Nanog

  • Positive control

    • Recombinant human Nanog.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    References (2)

    Publishing research using ab84231? Please let us know so that we can cite the reference in this datasheet.

    ab84231 has been referenced in 2 publications.

    • Friedman AD  et al. The smart targeting of nanoparticles. Curr Pharm Des 19:6315-29 (2013). PubMed: 23470005
    • Chikkaveeraiah BV  et al. Ultrasensitive nanostructured immunosensor for stem and carcinoma cell pluripotency gatekeeper protein NANOG. Nanomedicine (Lond) 7:957-65 (2012). PubMed: 22394186

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