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    biotin-nitro-tyrosine-antibody-ab27646.pdf

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Neuroscience Neurotransmission Nitric Oxide Nitrated Species
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Biotin Anti-Nitro tyrosine antibody (ab27646)

  • Datasheet
Submit a review Q&A (1)References (2)

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Abpromise

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Key features and details

  • Biotin Goat polyclonal to Nitro tyrosine
  • Conjugation: Biotin
  • Isotype: IgG

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Conjugation
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Overview

  • Product name

    Biotin Anti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description

    Biotin Goat polyclonal to Nitro tyrosine
  • Host species

    Goat
  • Conjugation

    Biotin
  • Immunogen

    Chemical/ Small Molecule (Nitrotyrosine)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 0.45% Sodium chloride, 0.02% EDTA disodium salt, 0.27% Monobasic dihydrogen sodium phosphate, 0.73% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purification notes

    Purified by nitrated protein-Sepharose™
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • Nitrated Species
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Platelets

Associated products

  • Isotype control

    • Biotin Goat IgG - Isotype Control (ab37376)

Applications

  • Application notes
    ELISA: Range 1/5000 - 1/40000.
    WB: Range 1/5000 - 1/40000. Predicted molecular weight: na kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
    • Alternative names

      • NO tyrosine antibody
      • nTyr antibody

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab27646? Please let us know so that we can cite the reference in this datasheet.

    ab27646 has been referenced in 2 publications.

    • Ferreira I  et al. Nitrated nucleosome levels and neuropsychiatric events in systemic lupus erythematosus; a multi-center retrospective case-control study. Arthritis Res Ther 19:287 (2017). PubMed: 29273092
    • Croca S  et al. Serum nitrated nucleosome levels in patients with systemic lupus erythematosus: a retrospective longitudinal cohort study. Arthritis Res Ther 16:R48 (2014). ELISA ; Human . PubMed: 24502558

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I have your product: Anti-Nitro tyrosine antibody (Biotin) (ab27646) which works perfect on my experiments. Now I want to buy the same antibody but NO Biotin on it. There are many goat Anti-Nitro tyrosine antibodies, so I don't know which one is the same as ab27646 but without biotin. Could you please the me the Cat. No which I want. Thank you very much!

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2012

    Answer

    Thank you for contacting us.

    The ab27628 Anti-Nitro tyrosine antibody is the one you are looking for:

    https://www.abcam.com/Nitro-tyrosine-antibody-ab27628.html (or use the following: https://www.abcam.com/Nitro-tyrosine-antibody-ab27628.html).


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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