Biotin Anti-Nitro tyrosine antibody (ab27646)
Key features and details
- Biotin Goat polyclonal to Nitro tyrosine
- Suitable for: ELISA, WB
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Nitro tyrosine antibody
See all Nitro tyrosine primary antibodies -
Description
Biotin Goat polyclonal to Nitro tyrosine -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Chemical/ Small Molecule (Nitrotyrosine)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.45% Sodium chloride, 0.02% EDTA disodium salt, 0.27% Monobasic dihydrogen sodium phosphate, 0.73% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purification notes
Purified by nitrated protein-Sepharose™ -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Target
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Relevance
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. -
Alternative names
- NO tyrosine antibody
- nTyr antibody
Datasheets and documents
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Datasheet download
References (2)
ab27646 has been referenced in 2 publications.
- Ferreira I et al. Nitrated nucleosome levels and neuropsychiatric events in systemic lupus erythematosus; a multi-center retrospective case-control study. Arthritis Res Ther 19:287 (2017). PubMed: 29273092
- Croca S et al. Serum nitrated nucleosome levels in patients with systemic lupus erythematosus: a retrospective longitudinal cohort study. Arthritis Res Ther 16:R48 (2014). ELISA ; Human . PubMed: 24502558
