Biotin Anti-Nitro tyrosine antibody (ab27646)
Key features and details
- Biotin Goat polyclonal to Nitro tyrosine
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Nitro tyrosine antibody
See all Nitro tyrosine primary antibodies -
Description
Biotin Goat polyclonal to Nitro tyrosine -
Host species
Goat -
Conjugation
Biotin -
Immunogen
Chemical/ Small Molecule (Nitrotyrosine)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.45% Sodium chloride, 0.02% EDTA disodium salt, 0.27% Monobasic dihydrogen sodium phosphate, 0.73% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purification notes
Purified by nitrated protein-Sepharose™ -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
WB: Range 1/5000 - 1/40000. Predicted molecular weight: na kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. -
Alternative names
- NO tyrosine antibody
- nTyr antibody
Datasheets and documents
References (2)
ab27646 has been referenced in 2 publications.
- Ferreira I et al. Nitrated nucleosome levels and neuropsychiatric events in systemic lupus erythematosus; a multi-center retrospective case-control study. Arthritis Res Ther 19:287 (2017). PubMed: 29273092
- Croca S et al. Serum nitrated nucleosome levels in patients with systemic lupus erythematosus: a retrospective longitudinal cohort study. Arthritis Res Ther 16:R48 (2014). ELISA ; Human . PubMed: 24502558
