Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)
![Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)](https://www.abcam.com/ps/products/183/ab183434/Images/ab183434-213446-anti-nmdar1-antibody-m308-48-biotin-western-blot.jpg "Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)")
Key features and details
- Biotin Mouse monoclonal [N308/48] to NMDAR1
- Suitable for: WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-NMDAR1 antibody [N308/48]
See all NMDAR1 primary antibodies -
Description
Biotin Mouse monoclonal [N308/48] to NMDAR1 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment within Rat NMDAR1 aa 1-400 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_058706.1
Database link: P35439 -
Positive control
- Rat brain tissue lysate
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General notes
The clone number has been updated from S308-48 to N308/48, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N308/48 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
1 µg/ml of ab183434 is sufficient for detection of NMDAR1 in 20µg of rat brain membrane lysate. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. 1 µg/ml of ab183434 is sufficient for detection of NMDAR1 in 20µg of rat brain membrane lysate. |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 24408 Rat
- SwissProt: P35439 Rat
- Unigene: 9840 Rat
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Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)Biotin Anti-NMDAR1 antibody [N308/48] (ab183434) at 1/1000 dilution + rat brain membrane lysate
Predicted band size: 105 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab183434 has not yet been referenced specifically in any publications.
