Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)

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Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)

    Key features and details

    • Biotin Mouse monoclonal [N308/48] to NMDAR1
    • Suitable for: WB
    • Reacts with: Rat
    • Conjugation: Biotin
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    HRP Unconjugated

    Overview

    • Product name

      Biotin Anti-NMDAR1 antibody [N308/48]
      See all NMDAR1 primary antibodies

    • Description

      Biotin Mouse monoclonal [N308/48] to NMDAR1

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      Recombinant fragment within Rat NMDAR1 aa 1-400 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_058706.1
      Database link: P35439

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Rat brain tissue lysate

    • General notes

      The clone number has been updated from S308-48 to N308/48, both clone numbers name the same antibody clone.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab183434 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.

    1 µg/ml of ab183434 is sufficient for detection of NMDAR1 in 20µg of rat brain membrane lysate.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.

    1 µg/ml of ab183434 is sufficient for detection of NMDAR1 in 20µg of rat brain membrane lysate.

    Target

    • Function

      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.

    • Post-translational
      modifications

      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt

    • Database links

      • Alternative names

        • GluN1 antibody
        • Glutamate [NMDA] receptor subunit zeta-1 antibody
        • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
        • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
        • glutamate receptor ionotropic, NMDA 1 antibody
        • Grin1 antibody
        • MRD8 antibody
        • N methyl D aspartate receptor antibody
        • N methyl D aspartate receptor channel subunit zeta 1 antibody
        • N methyl D aspartate receptor subunit NR1 antibody
        • N-methyl-D-aspartate receptor subunit NR1 antibody
        • NMD-R1 antibody
        • NMDA 1 antibody
        • NMDA R1 antibody
        • NMDA receptor 1 antibody
        • NMDA1 antibody
        • NMDAR antibody
        • NMDZ1_HUMAN antibody
        • NR1 antibody
        see all

      Images

      • Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)
        Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434)
        Biotin Anti-NMDAR1 antibody [N308/48] (ab183434) at 1/1000 dilution + rat brain membrane lysate

        Predicted band size: 105 kDa

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab183434? Please let us know so that we can cite the reference in this datasheet.

      ab183434 has not yet been referenced specifically in any publications.

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