Biotin Anti-PDGFR alpha antibody [APA5] (ab93530)

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Flow Cytometry - Biotin Anti-PDGFR alpha antibody [APA5] (ab93530)

    Key features and details

    • Biotin Rat monoclonal [APA5] to PDGFR alpha
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: Biotin
    • Isotype: IgG2a

    Overview

    • Product name

      Biotin Anti-PDGFR alpha antibody [APA5]
      See all PDGFR alpha primary antibodies

    • Description

      Biotin Rat monoclonal [APA5] to PDGFR alpha

    • Host species

      Rat

    • Conjugation

      Biotin

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Fusion protein corresponding to Mouse PDGFR alpha.

    • Positive control

      • NIH/3T3 cell line

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab93530 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.5-1µg for 105-8 cells.

    A final volume of 100 µl is recommended for staining the cell sample.

     

     

     

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.

    • Tissue specificity

      Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.

    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Alpha-type platelet-derived growth factor receptor antibody
        • CD140 antigen-like family member A antibody
        • CD140a antibody
        • CD140a antigen antibody
        • MGC74795 antibody
        • PDGF alpha chain antibody
        • PDGF Receptor alpha antibody
        • PDGF-R-alpha antibody
        • PDGFR 2 antibody
        • PDGFR alpha antibody
        • PDGFR2 antibody
        • PDGFRA antibody
        • PDGFRA/BCR fusion antibody
        • PGFRA_HUMAN antibody
        • Platelet derived growth factor receptor 2 antibody
        • Platelet derived growth factor receptor alpha antibody
        • Platelet derived growth factor receptor alpha polypeptide antibody
        • Platelet derived growth factor receptor antibody
        • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
        • RHEPDGFRA antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-PDGFR alpha antibody [APA5] (ab93530)
        Flow Cytometry - Biotin Anti-PDGFR alpha antibody [APA5] (ab93530)
        Staining of NIH/3T3 cell line with 0.5 µg of ab93530 followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

      References (2)

      Publishing research using ab93530? Please let us know so that we can cite the reference in this datasheet.

      ab93530 has been referenced in 2 publications.

      • Miyawaki T  et al. Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina. J Neurosci 24:8124-34 (2004). IHC-Fr . PubMed: 15371513
      • Takakura N  et al. PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. J Histochem Cytochem 45:883-93 (1997). IHC-P . PubMed: 9199674

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