Biotin Anti-Phosphoserine antibody (ab9335)
Key features and details
- Biotin Rabbit polyclonal to Phosphoserine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies -
Description
Biotin Rabbit polyclonal to Phosphoserine -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
BSA and KLH-phosphoserine conjugates.
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Positive control
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphoserine-agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. | |
| IP | Use at an assay dependent dilution. | |
| ELISA | Use a concentration of 0.5 µg/ml. |
Target
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Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. -
Alternative names
- phospho-Ser antibody
- pS antibody
- pSER antibody
Images
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Western blot - Biotin Anti-Phosphoserine antibody (ab9335)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
Protocols
Datasheets and documents
References (3)
ab9335 has been referenced in 3 publications.
- Zhang C et al. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep 7:3769 (2017). WB ; Human . PubMed: 28630428
- Granovski V et al. Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens. Intervirology 60:90-101 (2017). PubMed: 28957812
- Phillips JE et al. Glucocorticoid-induced osteogenesis is negatively regulated by Runx2/Cbfa1 serine phosphorylation. J Cell Sci 119:581-91 (2006). WB . PubMed: 16443755
