Key features and details
- Biotin Rabbit polyclonal to Phosphotyrosine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies
DescriptionBiotin Rabbit polyclonal to Phosphotyrosine
SpecificityRecognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
Tested applicationsSuitable for: WB, IP, ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin.
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.01% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography with phosphotyrosine-agarose.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
ab9331 has not yet been referenced specifically in any publications.