Biotin Anti-Phosphotyrosine antibody (ab9331)
Key features and details
- Biotin Rabbit polyclonal to Phosphotyrosine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies -
Description
Biotin Rabbit polyclonal to Phosphotyrosine -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins. -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin.
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Positive control
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphotyrosine-agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ELISA |
| Notes |
|---|
IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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Western blot - Biotin Anti-Phosphotyrosine antibody (ab9331)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab9331 has not yet been referenced specifically in any publications.
