Biotin Anti-Plasmin antibody (ab48350)

Reviews (1)Q&A (4)References (4)

Key features and details

  • Biotin Rabbit polyclonal to Plasmin
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-Plasmin antibody

  • Description

    Biotin Rabbit polyclonal to Plasmin

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Specificity

    This antibody does recognize plasminogen at about 20-40 %.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) - plasmin purified from human plasma.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Associated products

Target

  • Function

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

  • Tissue specificity

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.

  • Involvement in disease

    Plasminogen deficiency

  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.

  • Domain

    Kringle domains mediate interaction with CSPG4.

  • Post-translational
    modifications

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.

  • Cellular localization

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt

  • Database links

    • Alternative names

      • Plasmin light chain B antibody
      • Plasminogen antibody
      • PLG antibody
      • PLMN_HUMAN antibody

    References (4)

    Publishing research using ab48350? Please let us know so that we can cite the reference in this datasheet.

    ab48350 has been referenced in 4 publications.

    • Voegeli R  et al. Expression and ultrastructural localization of plasmin(ogen) in the terminally differentiated layers of normal human epidermis. Int J Cosmet Sci 41:624-628 (2019). PubMed: 31604367
    • Leksa V  et al. Dissecting mannose 6-phosphate/insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem : (2012). WB . PubMed: 22613725
    • Barker R  et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). WB ; Human . PubMed: 20709033
    • Sadagopan S  et al. Kaposi's sarcoma-associated herpesvirus upregulates angiogenin during infection of human dermal microvascular endothelial cells, which induces 45S rRNA synthesis, antiapoptosis, cell proliferation, migration, and angiogenesis. J Virol 83:3342-64 (2009). WB ; Human . PubMed: 19158252

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    Dear Sir or Madam, I am looking for a blocking antibody that would specifically inhibit human plasmin activity. I was not able to find one on your website but I may be wrong : do you indeed sell such antibodies? I would be very grateful if you would consider my request, I am looking from hearing from you at your earliest convenience, Yours sincerely,

    Read More
    Answer

    Thank you for contacting us.

    We currently offer only one anti-Plasmin antibody ab48350. This product has not been tested as a neutralizing/blocking antibody for plasmin. As a rabbit polyclonal this product will recognize a number of epitopes on plasmin and therefore may neutralize it, however there will also be lot to lot variation in epitope recognition.



    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    - about specifics of this antibody - why anti plasmin and not plasminogen?
    - has this antibody been tested against plasmin when complexed to PNA or PAI?

    Read More
    Answer

    Merci pour votre patience. Je m'excuse pour ce délais.

    Je peux confirmer que cet anticorps était produit contre la plasmine et reconnait aussi le plasminogène à environ 20 à 40% d'intensité.

    Je peux aussi confirmer que la reconnaissance des chaînes lourdes et légères n'a pas été testé séparément.

    Pour ce qui est de la reconnaissance de la plasmine complexé, le laboratoire n'as pas testé ceci et peux que faire des prédictions. Dans les deux cas c'est possible que cet anticorps reconnait la forme complexé, soit à la PAI soit la PNA complexé à travers des groupes carboxy.

    J'espère que ces informations vous sont toujours utiles. N'hésitez pas à nous recontacter si vous avez des autres questions.

    Veuillez aussi nous recontacter si vous êtes toujours intéressez de tester cet anticorps. Je pourrais éventuellement vous faire une offre de test.

    Read More

    Western blot abreview for Anti-Plasmin antibody (Biotin)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (Blood)
    Loading amount
    3 µg
    Specification
    Blood
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris HCl gels)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Rachel Barker

    Verified customer

    Submitted Aug 10 2009

    Question

    I have further questions regarding this product. Does this antibody only react to plasmin? Or does it also react to plasminogen? When you confirmed that this antibody only reacts to mouse plasmin 15%, does that mean the remainder 85% reacts to plasminogen? There is no amino acid difference between plasmin and plasminogen. Although antibodies are named plasmin, most of them will also react to plasminogen. Can you please confirm if this antibody will react to plasminogen?

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    Answer

    Thank you for your enquiry. This antibody has not been tested if they can recognize plasminogen. Therefore, I can not confirm if it only detects plasmin. This antibody reacts 15% to mouse sample, which means that the remaining 85% is no reaction.

    Read More

    Question

    What is the specificity for this product?

    Read More
    Answer

    Thank you for your enquiry. This antibody has been tested to full length human plasmin. It cross reacts with rat plasmin 10%, and mouse plasmin 15%.

    Read More

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