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RecombinantRabMAb

Recombinant Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Reviews (1)Q&A (5)References (14)
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol
  • Suitable for: IHC-P, Sandwich ELISA, WB, ELISA
  • Reacts with: Species independent
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]
    See all Polyethylene glycol primary antibodies

  • Description

    Biotin Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-P, Sandwich ELISA, WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BSA-PEG5K. IHC-P: Human cerebral cortex tissue

  • General notes

    The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing. 

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents in blocking buffer and antibody dilution buffer when performing ELISA using anti-PEG products.

    Learn more about PEG RabMAb® primary antibodies and kit - PEG RabMAb Product Portal Page

    If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.41 x 10 -9 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    PEG-B-47b

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet.
Sandwich ELISA
Use at an assay dependent concentration.
WB
1/50000. Predicted molecular weight: 2 kDa.

For unpurified use at 1/5000 - 1/20000.
ELISA (1)
Use a concentration of 1 µg/ml.

Coating plates for ELISA: 0 to 500 ng/ml.

For unpurified use at 0.5µg/ml, coating plates for ELISA: 5 to 10 ug/ml.
Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet.
Sandwich ELISA
Use at an assay dependent concentration.
WB
1/50000. Predicted molecular weight: 2 kDa.

For unpurified use at 1/5000 - 1/20000.
ELISA
Use a concentration of 1 µg/ml.

Coating plates for ELISA: 0 to 500 ng/ml.

For unpurified use at 0.5µg/ml, coating plates for ELISA: 5 to 10 ug/ml.

Target

  • Alternative names

    • PEG antibody

Images

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

    Biotin conjugate (ab53449) available for this clone.

  • Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Sandwich ELISA antigen dose-response curve using purified ab53449.

    Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

    Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution (purified) + BSA-PEG5K at 0.02 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 2 kDa
    Observed band size: 280 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue showing negative staining with purified ab53449 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Lane 1 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution (unpurified)
    Lane 2 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution (unpurified)
    Lane 3 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution (unpurified)

    All lanes : BSA-PEG

    Predicted band size: 2 kDa

  • Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (14)

Publishing research using ab53449? Please let us know so that we can cite the reference in this datasheet.

ab53449 has been referenced in 14 publications.

  • Sebastiani F  et al. Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles. ACS Nano N/A:N/A (2021). PubMed: 33754708
  • van Alem CMA  et al. Liposomal Delivery Improves the Efficacy of Prednisolone to Attenuate Renal Inflammation in a Mouse Model of Acute Renal Allograft Rejection. Transplantation 104:744-753 (2020). PubMed: 31929419
  • Stock AA  et al. Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes. Stem Cell Reports 14:91-104 (2020). PubMed: 31839542
  • Che L  et al. Computer-assisted engineering of programmed drug releasing multilayer nanomedicine via indomethacin-mediated ternary complex for therapy against a multidrug resistant tumor. Acta Biomater N/A:N/A (2019). PubMed: 31344512
  • Poirier N  et al. FR104, an antagonist anti-CD28 monovalent fab' antibody, prevents alloimmunization and allows calcineurin inhibitor minimization in nonhuman primate renal allograft. Am J Transplant 15:88-100 (2015). PubMed: 25488654
View all Publications for this product

Customer reviews and Q&As

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ELISA abreview for Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Human Serum (Processed blood sample.)
Specification
Processed blood sample.
Type
Sandwich (Detection)
Blocking step
BSA, Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 20 2014

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