RecombinantRabMAb

Recombinant Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)
  • Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [E61] to pro Caspase-3
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-pro Caspase-3 antibody [E61]
    See all pro Caspase-3 primary antibodies

  • Description

    Biotin Rabbit monoclonal [E61] to pro Caspase-3

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Specificity

    The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human pro Caspase-3 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: FFPE, normal human tonsil tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    E61

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • CASP3 antibody
      • Caspase 3 antibody
      • Caspase 3 apoptosis related cysteine peptidase antibody
      • CPP32 antibody
      • CPP32B antibody
      • Procaspase 3 antibody
      • SCA 1 antibody
      • SCA1 antibody
      • Yama antibody
      • Yama protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)

      IHC image of pro Caspase-3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with ab204632, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)
      Biotin Anti-pro Caspase-3 antibody [E61] (ab204632)

    References (0)

    Publishing research using ab204632? Please let us know so that we can cite the reference in this datasheet.

    ab204632 has not yet been referenced specifically in any publications.

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