Biotin Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab77310)

Submit a review Q&A (6)References (2)

Key features and details

  • Biotin Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-Prostate Specific Antigen antibody [A67-B/E3]
    See all Prostate Specific Antigen primary antibodies

  • Description

    Biotin Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Human

  • Immunogen

    30 kDa protein fraction prepared from human seminal plasma

  • General notes

    Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    References (2)

    Publishing research using ab77310? Please let us know so that we can cite the reference in this datasheet.

    ab77310 has been referenced in 2 publications.

    • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). PubMed: 25680117
    • Berry SM  et al. Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem 84:5518-23 (2012). Functional Studies ; Human . PubMed: 22632629

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Question

    Dear technical support team:

    This customer raises an enquiry about ab10184, ab77310 and ab119154, she wants to use these products to conduct the sELISA assay, therefore, she wants to know whether this experiment can work well with each other or not?

    If not, could you please offer any suggestion to her?

    Thanks for your kindly help

    Best regards

    Read More
    Answer

    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab77310 has never been tested in sandwich ELISA (sELISA). It has been tested in indirect ELISA only (ELISA). All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target so they do not interfere with the other antibody binding. Therefore, I regrettably we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA.

    Reviewing the details on the datasheet forab10184 Anti-Prostate Specific Antigen antibody [2H9], this has been tested in sELISA. Several matched pairs are suggested for use with this antibody:

    'Can be paired for Sandwich ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187) and Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).

    For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.'

    I can recommend to review the online datasheets for further information. I suggest it would be more appropriate for the customer to use one of these matched pairs suggested above with ab10184which we can guarantee for sandwich ELISA:

    ab10185
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html).

    ab10187
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-8A6-ab10187.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-8A6-ab10187.html).

    ab24466
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html).

    ab10185
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html).

    ab24466
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html).

    The ab119154 Prostate Specific Antigen proteinis of human origin and full length, and these antibodies are all tested and guarnateed for human proteins. So in theory it should work. However, please be aware this protein has not been tested and guaranteed in sELISA.

    I hope this will be helpful, and if you have any further questions, please do not hesitate to contact us.

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    Question

    Thanks for your kindly reply; this customer would like to conduct the non-conventional application which process is similar to ELISA application. I attached the purpose image in this letter; this customer wants to know whether this experiment can work well or not? Please check this and offer any suggestion to her.

    Thanks for your kindly help

    Best regards

    Read More
    Answer

    Thank you for your reply.

    I have reviewed the details of this experiment and have the following feedback:

    1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab102885.

    2. The ab102885 Easylink conjugation kit would be suitable for conjugating FITC to ab10187.

    3. In theory, the experiment the customer has described should work. However, these antibodies have not been tested in this type of application, an I am sorry we would not be able to guarantee them for this.

    4. Also, as discussed in the previous email, for sandwich ELISa type experiments, we would suggest to use a matched pair. ab10187 has been tested as a matched pair withthe following:

    Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185)
    Click here (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html).

    Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187)
    Click here (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-8A6-ab10187.html).

    Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).
    Click here (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html).
    (please note this is already HRP conjugated and therefore may not be suitable)

    For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.)

    These are unfortunately not biotin conjugated. However, we do have a biotin conjugation kits. I can recommend to review the online datasheets for further important information on how to use these kits:

    ab102864 EasyLink Biotin (type A) Conjugation Kit (3 x 100µg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html).

    ab102865 EasyLink Biotin (type A) Conjugation Kit (3 x 10µg Biotin)
    https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html (or use the following: https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html).

    ab102866 EasyLink Biotin (type B) Conjugation Kit (1 x 1mg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-1-x-1mg-Biotin-ab102866.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-1-x-1mg-Biotin-ab102866.html).

    ab102867 EasyLink Biotin (type B) Conjugation Kit (3 x 100µg Biotin)
    https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html (or use the following: https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html).

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Question

    Dear technical support team:
    This customer raised an enquire about ab77310 (Anti-Prostate Specific Antigen antibody [A67-B/E3] (Biotin)), this customer wants to used this antibody to conduct ELISA assay with ab119154 (Prostate Specific Antigen protein) and ab10184 (Anti-Prostate Specific Antigen antibody [2H9]), she wants to know whether this antibody can be a suitable detection antibody or not? Could you please help this customer to solve the problem?
    Thanks for your kindly help Best regards

    Read More
    Answer

    Thank you for your enquiry.

    Sandwich ELISA (sELISA) procedures can be difficult to optimize and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sELISA.

    The Prostate Specific Antigen antibody [2H9] ab10184 that your customer has found has been tested in sandwich ELISA.The information on the datasheet states that this can be paired for sELISA with one of the the following:

    Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185)
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-ab10185.html).

    Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187)
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-8A6-ab10187.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-8A6-ab10187.html).

    Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).
    https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html (or use the following: https://www.abcam.com/Prostate-Specific-Antigen-antibody-5A6-HRP-ab24466.html).

    For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.)

    I would suggest the customer selects a pair of antibodies for sELISAfrom the information above as ab77310 and ab119154 aretested in indirect ELISA only.They are not tested andguaranteedin sELISA.I would recommend using ab77310 or ab119154 in an indirect ELISA rather than sELISA.

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Question

    Hello, 1. I will be using this antibody to bind to prostate cancer cells from a prostate cancer patients whole blood. 2. The whole blood will first be enriched for prostate cancer cells via  a microfluidic device capturing via a mAb for a specific cell receptor. 3. Cells will be fixed, then ab77310 will be used to stain, and a secondary to that conjugated to a fluorophore will be used to detect via flourescent microsopy. 4. Let me know if you need more details. .com  

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    Answer

    DISCOUNT CODE:*****Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab77310 in ICC/IHC . This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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    Question

    I would like to test ab77310 in ICC/IF.

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    Answer

    I am very pleased to hear you would like to accept our offer and test ab77310 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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    Question

    I spoke to a customer who is waiting for a discount code for this product.

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    Answer

    I would be very happy to issue you a discount code for ab77310. Could you please let me know on application you will test it and on what type of tissue you will be using.

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