Key features and details
- Biotin Rabbit polyclonal to RFP
- Suitable for: WB
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-RFP antibody
See all RFP primary antibodies
DescriptionBiotin Rabbit polyclonal to RFP
Specificityab34771 recognises RFP. The antibody is predicted to react with all RFP variants from Discosoma (including DsRed, TdTomato, mOrange etc.) based on sequence homology and polyclonality of the antibody. However, such crossreactivity has not been QC tested.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Our Abpromise guarantee covers the use of ab34771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 27 kDa.|
RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
All lanes : Biotin Anti-RFP antibody (ab34771) at 1/2500 dilution
Lane 1 : 10 µg of HeLa cell
extract containing GFP
Lane 2 : 10 µg
of a HeLa lysate containing RFP recombinant protein.
All lanes : IRDye™800 conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Observed band size: 27 kDa why is the actual band size different from the predicted?
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
ab34771 staining mouse subcutaneous tissue that had been injected with a mixture of cells including mouse embryonic stem cells previously transduced with a retrovirus that expresses RFP.
The sample was fixed with paraformaldehyde and a heat mediated antigen retreival step using Sodium citrate 10 mM (pH 6.0) was performed. 1.5% serum was used as the blocking agent for 30 minutes at 20°C. The primary antibody was diluted 1/400 in 1xPBS with 1.5% normal serum and incubated for 1 hour at 20°C. A Biotinylated goat anti-rabbit IgG (H+L) antibody was used as the secondary.
Biotin Anti-RFP antibody (ab34771) at 1/1000 dilution
Peroxidase streptavidin secondary antibody at 1/40000 dilution
ab34771 staining mouse epidermis that had been damaged and injected with a mixture of cells inlcuding mouse embryonic stem cells previously transduced with a retrovirus that expresses RFP. The color is developed with DAB.
The sample was acetone fixed and incubated with ab34771 diluted 1/400 for 1 hour at 20°C. A Biotinylated goat anti-rabbit IgG (H+L) antibody was used as the secondary.
ab34771 has been referenced in 63 publications.
- Kwon J et al. Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy. Nat Commun 11:273 (2020). PubMed: 31937765
- Hill JL et al. Cortistatin-expressing interneurons require TrkB signaling to suppress neural hyper-excitability. Brain Struct Funct 224:471-483 (2019). PubMed: 30377803
- Sande-Melón M et al. Adult sox10+ Cardiomyocytes Contribute to Myocardial Regeneration in the Zebrafish. Cell Rep 29:1041-1054.e5 (2019). PubMed: 31644901
- MacKay H et al. DNA methylation in AgRP neurons regulates voluntary exercise behavior in mice. Nat Commun 10:5364 (2019). PubMed: 31792207
- Kothari P et al. Contractility kits promote assembly of the mechanoresponsive cytoskeletal network. J Cell Sci 132:N/A (2019). PubMed: 30559246