Biotin Anti-RFP antibody (ab34771)
Key features and details
- Biotin Rabbit polyclonal to RFP
- Suitable for: WB
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-RFP antibody
See all RFP primary antibodies -
Description
Biotin Rabbit polyclonal to RFP -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab34771 recognises RFP. The antibody is predicted to react with all RFP variants from Discosoma (including DsRed, TdTomato, mOrange etc.) based on sequence homology and polyclonality of the antibody. However, such crossreactivity has not been QC tested. -
Tested applications
Suitable for: WBmore details -
Immunogen
Fusion protein corresponding to RFP. Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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General notes
Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 27 kDa.
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| Notes |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 27 kDa. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - Biotin Anti-RFP antibody (ab34771)All lanes : Biotin Anti-RFP antibody (ab34771) at 1/2500 dilution
Lane 1 : 10 µg of HeLa cell
extract containing GFP
recombinant protein
Lane 2 : 10 µg
of a HeLa lysate containing RFP recombinant protein.
Secondary
All lanes : IRDye™800 conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Observed band size: 27 kDa why is the actual band size different from the predicted?
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. -
Western blot - Biotin Anti-RFP antibody (ab34771)Biotin Anti-RFP antibody (ab34771) at 1/1000 dilution
Secondary
Peroxidase streptavidin secondary antibody at 1/40000 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (75)
ab34771 has been referenced in 75 publications.
- Thorsen AS et al. Heterogeneity in clone dynamics within and adjacent to intestinal tumours identified by Dre-mediated lineage tracing. Dis Model Mech 14:N/A (2021). PubMed: 33093165
- Rhodes J et al. Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2. Nat Commun 12:705 (2021). PubMed: 33514716
- Brommer B et al. Improving hindlimb locomotor function by Non-invasive AAV-mediated manipulations of propriospinal neurons in mice with complete spinal cord injury. Nat Commun 12:781 (2021). PubMed: 33536416
- Grau N et al. Spatiotemporally controlled induction of gene expression in vivo allows tracking the fate of tumor cells that traffic through the lymphatics. Int J Cancer 147:1190-1198 (2020). PubMed: 31675122
- Kwon J et al. Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy. Nat Commun 11:273 (2020). PubMed: 31937765