Biotin Anti-S100 beta antibody (ab105371)

Reviews (1)Q&A (2)

Key features and details

  • Biotin Rabbit polyclonal to S100 beta
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-S100 beta antibody
    See all S100 beta primary antibodies

  • Description

    Biotin Rabbit polyclonal to S100 beta

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human S100 beta protein purified from Human plasma.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

  • Tissue specificity

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

  • Sequence similarities

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • NEF antibody
      • Protein S100 B antibody
      • Protein S100-B antibody
      • S 100 calcium binding protein beta chain antibody
      • S 100 protein beta chain antibody
      • S-100 protein beta chain antibody
      • S-100 protein subunit beta antibody
      • S100 antibody
      • S100 calcium binding protein beta (neural) antibody
      • S100 calcium-binding protein B antibody
      • S100 protein beta chain antibody
      • S100B antibody
      • S100B_HUMAN antibody
      • S100beta antibody
      see all

    References (0)

    Publishing research using ab105371? Please let us know so that we can cite the reference in this datasheet.

    ab105371 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    I am trying to build an ELISA assay for S100b, but i need the primary antibody to be biotinylated so that it can be anchored to the streptavidin base.  So, is the product Anti-S100 beta antibody (Biotin) (ab105371)/Biotin? If yes, what is its binding capacity or activity measure? Thank you.

    Read More
    Answer

    Thank you for contacting us.

    I do wish to apologize for the delay in getting this information to you. Ab105371, Anti-S100 beta antibody, is a Biotinylated antibody. The molar ratio is between 2 – 5 biotin molecules per IgG protein.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Western blot abreview for Anti-S100 beta antibody (Biotin)

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (HEK and Mouse hippocapus)
    Gel Running Conditions
    Reduced Denaturing (15)
    Loading amount
    20 µg
    Specification
    HEK and Mouse hippocapus
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 03 2017

    Question

    Customer Services and Scientific Support Team,
    Thanks you for your reply. However, there are two issues:
    1. Following the link you provided for product no. ab95190 led me to a
    message saying that the product is no longer available in Abcam's
    product range. Do you have any recommendation as to an alternative for
    the S100 standard protein, for use with ab8330?
    2. It looks like for my application, I will need to amplify my sELISA
    signal using a biotinylated system. I'm considering other antibodies
    in your product range which have not been certified by Abcam for
    sELISA, to use as detection antibody. Are you able to provide a small
    amount of sample antibody for testing, say product no. ab48653 or
    ab105371? I intend to go with ab8330 as capture antibody.
    Many thanks for your help again.
    Best wishes,

    Read More
    Answer

    Thank you for your reply.

    My sincere apologies for the confusion. In this case, I am sorry we do not have a suitable standard protein to suggest on this occasion.

    In answer to your second question regarding ab48653 and ab105371, I am sorry these have not been tested in sandwich ELISA and regrettably would not be covered by the guarantee for this application. They have been tested only in indirect ELSA for each antibody against its antigen.

    I am sorry to confirm that because we carry over 90 000 products, it is not possible for use tostock sample vials to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again by replying to this email prior to purchase if you would like more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contactme again with any other questions

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