Biotin Anti-SIRP alpha antibody [OX-41] (ab33987)
Key features and details
- Biotin Mouse monoclonal [OX-41] to SIRP alpha
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-SIRP alpha antibody [OX-41]
See all SIRP alpha primary antibodies -
Description
Biotin Mouse monoclonal [OX-41] to SIRP alpha -
Host species
Mouse -
Conjugation
Biotin -
Species reactivity
Reacts with: Rat -
Immunogen
Peritoneal macrophages (Rat)
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Epitope
This clone binds to a different epitope on CD172a than ED 9. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
OX-41 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Application notes
Flow Cyt: Use neat. Use 10ul to label 106 cells in 100ul.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. This product requires protein digestion pre-treatment of paraffin sections, eg.trypsin or pronase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. This product requires protein digestion pre-treatment of paraffin sections, eg.trypsin or pronase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25528 Rat
- SwissProt: P97710 Rat
- Unigene: 53971 Rat
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab33987 has been referenced in 2 publications.
- Adams S et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol 161:1853-9 (1998). PubMed: 9712053
- Robinson AP et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47 (1986). PubMed: 3512425