Biotin Anti-SIRP alpha antibody [OX-41] (ab33987)
Key features and details
- Biotin Mouse monoclonal [OX-41] to SIRP alpha
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG2a
Overview
-
Product name
Biotin Anti-SIRP alpha antibody [OX-41]
See all SIRP alpha primary antibodies -
Description
Biotin Mouse monoclonal [OX-41] to SIRP alpha -
Host species
Mouse -
Conjugation
Biotin -
Species reactivity
Reacts with: Rat -
Immunogen
Peritoneal macrophages (Rat)
-
Epitope
This clone binds to a different epitope on CD172a than ED 9. -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
OX-41 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. This product requires protein digestion pre-treatment of paraffin sections, eg.trypsin or pronase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 25528 Rat
- SwissProt: P97710 Rat
- Unigene: 53971 Rat
-
Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Protocols
Datasheets and documents
References (2)
ab33987 has been referenced in 2 publications.
- Adams S et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol 161:1853-9 (1998). PubMed: 9712053
- Robinson AP et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47 (1986). PubMed: 3512425
