Key features and details
- Biotin Rat monoclonal [P84] to SIRP alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG1
Product nameBiotin Anti-SIRP alpha antibody [P84]
See all SIRP alpha primary antibodies
DescriptionBiotin Rat monoclonal [P84] to SIRP alpha
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Membrane fractions from Mouse brain.
- Mouse C57BL/6 bone marrow cells
ab95644 has been reported to have neutralizing activity.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 2.68% PBS
Concentration information loading...
PurityProtein G purified
Primary antibody notesab95644 has been reported to have neutralizing activity.
Light chain typekappa
Our Abpromise guarantee covers the use of ab95644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.125-0.25µg for 105-8 cells.
A final volume of 100 µl is recommended for staining the cell sample.
ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
- Information by UniProt
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
ab95644 has been referenced in 2 publications.
- Fukunaga A et al. Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes. J Immunol 172:4091-9 (2004). Flow Cyt . PubMed: 15034021
- Chuang W & Lagenaur CF Central nervous system antigen P84 can serve as a substrate for neurite outgrowth. Dev Biol 137:219-32 (1990). PubMed: 2303162