Biotin Anti-SIRP alpha antibody [P84] (ab95644)
![Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (ab95644)](https://www.abcam.com/ps/products/95/ab95644/Images/ab95644-109912-anti-sirp-alpha-antibody-p84-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (ab95644)")
Key features and details
- Biotin Rat monoclonal [P84] to SIRP alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-SIRP alpha antibody [P84]
See all SIRP alpha primary antibodies -
Description
Biotin Rat monoclonal [P84] to SIRP alpha -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse SIRP alpha. Membrane fractions from Mouse brain.
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Positive control
- Mouse C57BL/6 bone marrow cells
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General notes
ab95644 has been reported to have neutralizing activity.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab95644 has been reported to have neutralizing activity. -
Clonality
Monoclonal -
Clone number
P84 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.125-0.25µg for 105-8 cells.
A final volume of 100 µl is recommended for staining the cell sample.
ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µl is recommended for staining the cell sample.
ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 19261 Mouse
- SwissProt: P97797 Mouse
- Unigene: 1682 Mouse
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
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Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (ab95644)Staining of C57BL/6 bone marrow cells with with Anti-Mouse CD11b APC and 0.125 µg of ab95644, follwed by Streptavidin PE. Cells in the lymphoid (left) or myeloid (right) gates were used for analysis.
Datasheets and documents
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Datasheet download
References (2)
ab95644 has been referenced in 2 publications.
- Fukunaga A et al. Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes. J Immunol 172:4091-9 (2004). Flow Cyt . PubMed: 15034021
- Chuang W & Lagenaur CF Central nervous system antigen P84 can serve as a substrate for neurite outgrowth. Dev Biol 137:219-32 (1990). PubMed: 2303162
