For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    biotin-sodium-potassium-atpase-antibody-ep1845y-plasma-membrane-loading-control-ab198366.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Plasma Membrane
Share by email
RecombinantRabMAb

Recombinant Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
  • Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free HRP PE Unconjugated

You may also be interested in

Primary
Product image
Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (ab167390)
Conjugation
Product image
Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
IHC
Product image
Streptavidin HRP (ready-to-use) (ab64269)

View more associated products

Overview

  • Product name

    Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
    See all Sodium Potassium ATPase primary antibodies
  • Description

    Biotin Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Tilapia
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: normal human cerebral cortex tissue sections
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1845Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (ab167390)
    • HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)
    • Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab197713)
    • Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab198367)
    • PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab209299)
    • Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab210143)
    • Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)
    • Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P05023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 476 Human
    • Entrez Gene: 480 Human
    • Entrez Gene: 481 Human
    • Entrez Gene: 11928 Mouse
    • Entrez Gene: 11931 Mouse
    • Entrez Gene: 27222 Mouse
    • Entrez Gene: 24211 Rat
    • Entrez Gene: 25650 Rat
    • Entrez Gene: 29132 Rat
    • Omim: 182310 Human
    • Omim: 182330 Human
    • Omim: 607321 Human
    • SwissProt: P05023 Human
    • SwissProt: P05026 Human
    • SwissProt: Q13733 Human
    • SwissProt: P14094 Mouse
    • SwissProt: Q8VDN2 Mouse
    • SwissProt: Q9WV27 Mouse
    • SwissProt: P06685 Rat
    • SwissProt: P07340 Rat
    • SwissProt: Q64541 Rat
    • Unigene: 371889 Human
    • Unigene: 280103 Mouse
    • Unigene: 217534 Rat
    • Unigene: 2992 Rat
    see all
  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)

    IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab198366, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
    Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab198366? Please let us know so that we can cite the reference in this datasheet.

ab198366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab198366.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.