Biotin Anti-Staphylococcus aureus antibody (ab35192)

DISCOUNT CODE: ***
Expiration date: March 10, 2013
Value: $424

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Thank you for your reply.

I have attached the Certificate of Compliance for ab34295 (lot **-1).

Please let me know if there is anything else I can help you with.

Thank you for contacting Abcam.

I have attached the datasheet for ab35192, is that what you need? If you require a Certificate of Compliance, then please let me know and I will have one generated for you.

Please let me know if there is anything else I can help you with.

Thank you for your response and follow up inquiry. Protein A on the surface will interact non-specifically with this antibody, as well as other Ig isotypes which can interact with Protein A. If you would like to minimize this interaction, you can block their sample with normal serum prior to primary antibody incubation. Normal serum from the species of the secondary antibody would be best. 10% normal serum incubated for about 10-15 minutes should help.

Thank you for contacting us.

Thank you for your inquiry regarding 0300-0085. For this specific antibody, the method of purification is proprietary but I can say that it is greater than 95% purity. The antibody has been tested against both soluble and structural antigens of the whole bacterium, so it is expected to react with Protein A.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

Just to clarify, you have 1 vial labeled ab35192 and the other labeled ab35192-1? You had listed ab35912 which is a Histone H1.4 peptide no longer available on the catalogue, so I think this was just a typo.

The -1 just signifies the size (1 mL). This was an older method of labeling that we used to employ, but newer labels have dropped the size from the catalogue number so you won't see the -1 anymore (it was confusing to customers). But yes, they are the same product.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The anti-Staphylococcus aureus antibodies ab68954, ab35192 and ab20002 have not all been tested in ELISA and cannot therefore be guaranteed in this application. Ab35192 has currently only been used in ICC/IF but this does not mean that it would not work in this application, simply that we have not tested it and do not know if it will be suitable.

With the Anti-Staphylococcus aureus antibody [702] (ab20002), the antibody was used in a direct ELISA protocol using the heat killed Salmonella organisms directly to the plate. The cells were not lysed prior to coating on the plate. The Staph. aureus cells (5 x 107/ml) were bound to the wells of Immulon II 96-well plates in 50ul of 100 mM carbonate buffer, pH 9.6, per well.The plates are sealed with plastic wrap and incubated overnight at room temperature. The next day, the coating solution is removed by inverting the plates. The wellswere then blocked for 30 minutes with 1% BSA-PBS-0.05% Tween 20. This solution was then removed by inverting the plates.The coated plates are then ready for testing with the antibody.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact us again.

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Please find below the information I managed to collect by contacting the sources of the Anti-Staphylococcus aureusand the Anti-Streptococcus agalactiaeantibodies we have in our catalog :

Anti-Staphylococcus aureus:
https://www.abcam.com/ab23568 shows no reactivity to any streptococcus sp., mycoplasma sp. or corynebacteria sp.

Unfortunately, ab35192 has not been specifically tested against streptococcus so we are unable to guarantee it won’t react to S. agalactiae.

ab68950, ab68952, ab68954: These Staphylococcus aureus polyclonal antibodies are not absorbed for related microorganisms and so therefore could cross react with related bacteria such as Staph. Epidermidis.

MLS : Staphylococcus aureus: We do not have an antibody that is specific to Staph. aureus but does not have crossreactivity to Staph. epidermidis.

Anti-Streptococcus agalactiae:
https://www.abcam.com/ab21126 has weak crossreactivity with Strep. dysgalactiae and does not cross-react with Strep. uberis, Strep. bovis, Strep. faecalis, Staph. aureus, Mycoplasma bovis, Mycoplasma californicum, Mycoplasma bovigenitalium, E. coli or Corynebacterium bovis.

I hope this information is helpful to you. I am still waiting for more information about ab128711 (Anti-Streptococcus agalactiae antibody [224/46]).
Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply.

We only have the full size vials, but we do fully guarantee both of these antibodies to work as stated on the datasheets. If the results were unsatisfactory with one I would be happy to send the other free of charge.

Please let me know if you have any further questions or if there is anything else we might be able to do for you.

Thank you for your call today and for your questions about ab35192 and ab68954. It was a pleasure speaking to you.
I've heard back from the lab, and the immunogen for this Staphylococcus aureus antibody is cultured Staph, ATCC 27660, whichare not lysed. Unfortunately we have not done any affinity analysis for the surface epitopes, so we do not have any further information about this.

I hope this information will be useful, but if there is anything else that we can do for you, please don't hesitate to ask.