Biotin Anti-Staphylococcus aureus antibody (ab35192)
Key features and details
- Biotin Rabbit polyclonal to Staphylococcus aureus
- Suitable for: ICC/IF
- Reacts with: Staphylococcus aureus
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Staphylococcus aureus antibody
See all Staphylococcus aureus primary antibodies -
Description
Biotin Rabbit polyclonal to Staphylococcus aureus -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
Reacts with soluble and structural antigens of the whole bacterium. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Tissue, cells or virus corresponding to Staphylococcus aureus. Native Staphylococcus aureus, ATCC 27660.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified IgG conjugated to Biotin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent dilution.
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Notes |
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ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall. -
Database links
- Entrez Gene: 3919448 Staphylococcus aureus
- SwissProt: P02976 Staphylococcus aureus
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Alternative names
- IgG binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab35192 has been referenced in 3 publications.
- Smith S et al. Anti-Escherichia coli Functionalized Silver-Doped Carbon Nanofibers for Capture of E. coli in Microfluidic Systems. Polymers (Basel) 12:N/A (2020). PubMed: 32414196
- Veli M & Ozcan A Computational Sensing of Staphylococcus aureus on Contact Lenses Using 3D Imaging of Curved Surfaces and Machine Learning. ACS Nano 12:2554-2559 (2018). PubMed: 29522316
- Diep BA et al. Identifying potential therapeutic targets of methicillin-resistant Staphylococcus aureus through in vivo proteomic analysis. J Infect Dis 209:1533-41 (2014). PubMed: 24280367