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  1. Link

    biotin-streptococcus-group-a-antibody-ab20509.pdf

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Microbiology Organism Bacteria Gram positive bacteria Streptococcus
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Biotin Anti-Streptococcus group A antibody (ab20509)

  • Datasheet
  • SDS
Submit a review Q&A (1)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Biotin Goat polyclonal to Streptococcus group A
  • Suitable for: ELISA, ICC/IF
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-Streptococcus group A antibody
    See all Streptococcus group A primary antibodies
  • Description

    Biotin Goat polyclonal to Streptococcus group A
  • Host species

    Goat
  • Conjugation

    Biotin
  • Specificity

    Type specific carbohydrate of group A Streptococcus.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with Streptococcus.
  • Immunogen

    Streptococci, group A.

  • General notes

    The antibody is covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Streptococcus

Associated products

  • Isotype control

    • Biotin Goat IgG - Isotype Control (ab37376)

Applications

Our Abpromise guarantee covers the use of ab20509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nose and pharynx. Streptococci can be divided into many groups on the basis of antigenic differences in group-specific polysaccharides located in the bacterial cell wall. More than 20 serologic groups have been identified and designated by letters, eg, A, B, C. Group A streptococci, cause a wide-range of disease in humans, from mild sore throats to life-threatening invasive disease such as necrotising fasciitis. Streptococcus pyogenes (a group A Streptococcus) is one of the most important pathogens encountered in clinical practice. Group A streptococci (GAS) are gram-positive, nonmotile, non-spore-forming organisms that appear as pairs or short-to-moderate-sized chains. Group A organisms can be identified by enzyme immunoassays.
    • Alternative names

      • Group A strep antibody
      • Group A streptococci antibody
      • Group A streptococcus antibody

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab20509? Please let us know so that we can cite the reference in this datasheet.

    ab20509 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    ab25870
    Please confirm which strain of Strep group A this was raised against.
    Please provide a list of the strains in which other Strep group A antibodies have been tested.
    Using WB and in vivo.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 05 2013

    Answer

    Thank you for your recent telephone enquiry.

    I am sorry it has taken some time ot obtain the required information for you as I had quite a few people to contact to gather the details.

    I obtained information on the following Strep group A antibodies that have been tested in western blot. As discussed on the telephone, I am sorry to confirm that these will not have been tested in vivo.

    ab20509 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=20509 (or use the following: https://www.abcam.com/index.html?datasheet=20509).

    ab20752 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=20752 (or use the following: https://www.abcam.com/index.html?datasheet=20752).

    ab20915 Positive for Streptococcus pyogenes (ATCC 12344, UV-inactivated), Negative for Group B: Type 1a (ATCC 12400), Type 1b (ATCC 12401), Type 1c (ATCC 27591), Type II (ATCC 12973) and Type III (ATCC 12403); Group C = ATCC 12960; Group D = ATCC 12389; Group E = ATCC 12390, Group F = ATCC 12393 & Group G = ATCC 27961.
    https://www.abcam.com/index.html?datasheet=20915 (or use the following: https://www.abcam.com/index.html?datasheet=20915).


    ab21034 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=21034 (or use the following: https://www.abcam.com/index.html?datasheet=21034).

    ab21046 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=21046 (or use the following: https://www.abcam.com/index.html?datasheet=21046).

    ab25870 Specific for Group A carbohydrate.
    https://www.abcam.com/index.html?datasheet=25870 (or use the following: https://www.abcam.com/index.html?datasheet=25870).


    ab36303 Anti-Streptococcus group A antibody [5A1]
    https://www.abcam.com/index.html?datasheet=36303 (or use the following: https://www.abcam.com/index.html?datasheet=36303).
    This antibody was tested and found positive against the following:
    a. Group A Streptococcus = Streptococcus pyogenes, ATCC #12344

    2. The antibody was tested and found negative against the following:

    a. Group B Streptococcus = Type Ia (ATCC # 12400), Type Ib (ATCC # 12401), Type Ic (ATCC #27591), Type II (ATCC # 12973), and Type III (ATCC# 12403)

    b. Group C Streptococcus = ATCC # 12960

    c. Group D Streptococcus = ATCC #12389

    d. Group E Streptococcus = ATCC # 12390

    e. Group F Streptococcus = ATCC #12393

    f. Group G Streptococcus = ATCC #27961


    ab68879 ab68880 ab68881 have been specifically tested for reactivity against the ATCC 10403 strain of Group A Streptococcus. It was also tested and found to be negative against Treponema ryder, S. pneumoniae, and Streptococcus Group B, C, D, F, and G.
    https://www.abcam.com/index.html?datasheet=68879 (or use the following: https://www.abcam.com/index.html?datasheet=68879).
    https://www.abcam.com/index.html?datasheet=68880 (or use the following: https://www.abcam.com/index.html?datasheet=68880).
    https://www.abcam.com/index.html?datasheet=68881 (or use the following: https://www.abcam.com/index.html?datasheet=68881).

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Feb 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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