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Microbiology Organism Bacteria Gram negative Bacteria Mycoplasma
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Biotin Anti-TB Ag85A antibody (ab193509)

  • Datasheet
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Western blot - Biotin Anti-TB Ag85A antibody (ab193509)

    Key features and details

    • Biotin Rabbit polyclonal to TB Ag85A
    • Suitable for: WB
    • Conjugation: Biotin
    • Isotype: IgG

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    Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)
    Primary
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    Anti-TB Ag85A antibody (ab193499)
    Conjugation
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    Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)

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    Overview

    • Product name

      Biotin Anti-TB Ag85A antibody
      See all TB Ag85A primary antibodies
    • Description

      Biotin Rabbit polyclonal to TB Ag85A
    • Host species

      Rabbit
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant fragment corresponding to TB Ag85A aa 53-331. Mycobacterium tuberculosis. (P9WQP2, P9WQP3).
      Sequence:

      YLQVPSPS MGRDIKVQFQ SGGANSPALY LLDGLRAQDD FSGWDINTPA FEWYDQSGLS VVMPVGGQSS FYSDWYQPAC GKAGCQTYKW ETFLTSELPG WLQANRHVKP TGSAVVGLSM AASSALTLAI YHPQQFVYAG AMSGLLDPSQ AMGPTLIGLA MGDAGGYKAS DMWGPKEDPA WQRNDPLLNV GKLIANNTRV WVYCGNGKPS DLGGNNLPAK FLEGFVRTSN IKFQDAYNAG GGHNGVFDFP DSGTHSWEYW GAQLNAMKPD LQRALGATPN T

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • TB Ag85A protein.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
    • Concentration information loading...
    • Purity

      Caprylic Acid - Ammonium Sulfate precipitation
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Mycoplasma

    Associated products

    • Isotype control

      • Biotin Rabbit IgG - Isotype Control (ab200208)
    • Recombinant Protein

      • Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)
    • Related Products

      • Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)

    Applications

    Our Abpromise guarantee covers the use of ab193509 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 2 µg/ml. Predicted molecular weight: 36 kDa.

    Target

    • Relevance

      Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
    • Cellular localization

      Secreted; cell wall. Cytoplasm
    • Alternative names

      • 85 A antibody
      • 85A antibody
      • Ag85A antibody
      • Antigen 85 A antibody
      • Antigen 85 complex A antibody
      • Antigen 85A antibody
      • Fbp A antibody
      • FbpA antibody
      • Fibronectin binding protein A antibody
      • Mpt 44 antibody
      • Mpt44 antibody
      • Mycobacterium tuberculosis antigen 85A antibody
      • Mycolyl transferase 85 A antibody
      • Mycolyl transferase 85A antibody
      • Rv3804c antibody
      • Secreted antigen Ag85A antibody
      see all

    Images

    • Western blot - Biotin Anti-TB Ag85A antibody (ab193509)
      Western blot - Biotin Anti-TB Ag85A antibody (ab193509)
      All lanes : Biotin Anti-TB Ag85A antibody (ab193509) at 2 µg/ml

      Lane 1 : Recombinant Mycobacterium tuberculosis Antigen 85-A protein at 1 µg
      Lane 2 : Recombinant Mycobacterium tuberculosis Antigen 85-A protein at 10 µg

      Predicted band size: 36 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab193509? Please let us know so that we can cite the reference in this datasheet.

    ab193509 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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