Biotin Anti-Thy1.1 antibody [HIS51] (ab95811)
![Flow Cytometry - Biotin Anti-Thy1.1 antibody [HIS51] (ab95811)](https://www.abcam.com/ps/products/95/ab95811/Images/ab95811-110055-anti-thy11-antibody-his51-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-Thy1.1 antibody [HIS51] (ab95811)")
Key features and details
- Biotin Mouse monoclonal [HIS51] to Thy1.1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Thy1.1 antibody [HIS51]
See all Thy1.1 primary antibodies -
Description
Biotin Mouse monoclonal [HIS51] to Thy1.1 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Rat bone marrow cells
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Positive control
- Rat thymocyte and splenocyte suspensions
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HIS51 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab95811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.03-0.06µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. -
Cellular localization
Attached to the membrane by a GPI anchor. -
Database links
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
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Alternative names
- CD90 antibody
- CD90.1 antibody
- CDw90 antibody
see all
Images
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Flow Cytometry - Biotin Anti-Thy1.1 antibody [HIS51] (ab95811)Flow cytometric analysis of Rat splenocytes with 0.03 µg of Anti-Mouse/Rat CD90.1 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Datasheets and documents
References (0)
ab95811 has not yet been referenced specifically in any publications.
