Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

Submit a review Q&A (1)References (4)
Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
  • Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

Key features and details

  • Biotin Mouse monoclonal [TL2.1] to TLR2
  • Suitable for: IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-TLR2 antibody [TL2.1]
    See all TLR2 primary antibodies

  • Description

    Biotin Mouse monoclonal [TL2.1] to TLR2

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset

  • Immunogen

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • General notes

    Ab9101 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab9101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Post-translational
    modifications

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
    Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

    Immunohistochemistry analysis of human brain frozen tissue tissue labelling TLR2 with ab9101

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

    Immunohistochemical analysis of human TLR2 in paraffin-embedded human spleen tissue.

  • Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
    Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
    TLR2 staining on human peripheral blood mononuclear cells by using ab9101. Green: CD3+-cells; Red: CD14+ cells.

References (4)

Publishing research using ab9101? Please let us know so that we can cite the reference in this datasheet.

ab9101 has been referenced in 4 publications.

  • Zhang P  et al. Toll-Like Receptors 2 and 4 Predict New-Onset Atrial Fibrillation in Acute Myocardial Infarction Patients. Int Heart J 59:64-70 (2018). PubMed: 29375116
  • Flo TH  et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 69:474-81 (2001). PubMed: 11261796
  • Wang JE  et al. Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 69:2402-6 (2001). PubMed: 11254600
  • Flo TH  et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 164:2064-9 (2000). PubMed: 10657659

Customer reviews and Q&As

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Question

I am interested in your TLR2 antibody for Western blotting. Can you give me some information about what target cells you used and what the conditions were for this assay.

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Answer

The following article describes the use of HM2064 (monoclonal antibody against human TLR-2, clone TL2.1): Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NK-kappa B activation. E.Faure et al. J. Immunol 2001, 166 p. 2018.

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