Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
![Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)](https://www.abcam.com/ps/products/9/ab9101/Images/ab9101-451274-biotin-anti-tlr2-antibody-immunohistochemistry-frozen-sections-human-brain.jpg "Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)")
Key features and details
- Biotin Mouse monoclonal [TL2.1] to TLR2
- Suitable for: IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TLR2 antibody [TL2.1]
See all TLR2 primary antibodies -
Description
Biotin Mouse monoclonal [TL2.1] to TLR2 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.
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General notes
Ab9101 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Primary antibody notes
Ab9101 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells. -
Clonality
Monoclonal -
Clone number
TL2.1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Post-translational
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7097 Human
- Omim: 603028 Human
- SwissProt: O60603 Human
- Unigene: 519033 Human
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Alternative names
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
Immunohistochemistry analysis of human brain frozen tissue tissue labelling TLR2 with ab9101
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)](https://www.abcam.com/ps/products/9/ab9101/Images/ab9101-282307-anti-tlr2-antibody-tl21-biotin-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)Immunohistochemical analysis of human TLR2 in paraffin-embedded human spleen tissue.
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![Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)](https://www.abcam.com/ps/products/9/ab9101/Images/ab9101-16778-anti-tlr2-antibody-tl21-biotin-flow-cytometry.jpg)
Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)TLR2 staining on human peripheral blood mononuclear cells by using ab9101. Green: CD3+-cells; Red: CD14+ cells.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab9101 has been referenced in 8 publications.
- Bridi M et al. Transcriptional corepressor SIN3A regulates hippocampal synaptic plasticity via Homer1/mGluR5 signaling. JCI Insight 5:N/A (2020). PubMed: 32069266
- Zhao Q et al. Xiaozhang Tie Improves Intestinal Motility in Rats With Cirrhotic Ascites by Regulating the Stem Cell Factor/c-kit Pathway in Interstitial Cells of Cajal. Front Pharmacol 11:1 (2020). PubMed: 32116689
- Steinberger J et al. Tracing MYC Expression for Small Molecule Discovery. Cell Chem Biol 26:699-710.e6 (2019). PubMed: 30880156
- Howard L et al. CD40 forward signalling is a physiological regulator of early sensory axon growth. Development 146:N/A (2019). PubMed: 31488565
- Zhang P et al. Toll-Like Receptors 2 and 4 Predict New-Onset Atrial Fibrillation in Acute Myocardial Infarction Patients. Int Heart J 59:64-70 (2018). PubMed: 29375116
- Flo TH et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 69:474-81 (2001). PubMed: 11261796
- Wang JE et al. Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 69:2402-6 (2001). PubMed: 11254600
- Flo TH et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 164:2064-9 (2000). PubMed: 10657659
