Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)
![Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)](https://www.abcam.com/ps/products/17/ab17264/Images/ab17264-282315-anti-tlr3-antibody-tlr37-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)")
Key features and details
- Biotin Mouse monoclonal [TLR3.7] to TLR3
- Suitable for: Flow Cyt
- Reacts with: Mouse, Dog, Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-TLR3 antibody [TLR3.7]
See all TLR3 primary antibodies -
Description
Biotin Mouse monoclonal [TLR3.7] to TLR3 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Dog, Human -
Immunogen
Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.
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Positive control
- Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
TLR3.7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab17264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
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Alternative names
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
Images
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Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)
Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab17264 at 10 μg/ml.
Datasheets and documents
References (2)
ab17264 has been referenced in 2 publications.
- Matsumoto M et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . PubMed: 12960343
- Matsumoto M et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). PubMed: 12054664
