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    biotin-tlr3-antibody-tlr37-ab17264.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)

  • Datasheet
Submit a review Q&A (1)References (2)

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Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)

    Key features and details

    • Biotin Mouse monoclonal [TLR3.7] to TLR3
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Dog, Human
    • Conjugation: Biotin
    • Isotype: IgG1

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    Recombinant human TLR3 protein (ab73825)

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    Overview

    • Product name

      Biotin Anti-TLR3 antibody [TLR3.7]
      See all TLR3 primary antibodies
    • Description

      Biotin Mouse monoclonal [TLR3.7] to TLR3
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse, Dog, Human
    • Immunogen

      Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

    • Positive control

      • Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      0.2 µm filtered
    • Clonality

      Monoclonal
    • Clone number

      TLR3.7
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Immunoglobulins
      • Receptors
      • Immunology
      • Innate Immunity
      • Chemokines
      • Beta Chemokines (CC)
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Alternative Versions

      • Anti-TLR3 antibody [TLR3.7] (ab12085)
    • Isotype control

      • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
    • Recombinant Protein

      • Recombinant human TLR3 protein (ab73825)

    Applications

    Our Abpromise guarantee covers the use of ab17264 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/50.

    ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity

      Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
    • Involvement in disease

      Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.
    • Domain

      ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
    • Post-translational
      modifications

      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
    • Cellular localization

      Endoplasmic reticulum membrane. Endosome membrane.
    • Target information above from: UniProt accession O15455 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7098 Human
      • Entrez Gene: 142980 Mouse
      • Omim: 603029 Human
      • SwissProt: O15455 Human
      • SwissProt: Q99MB1 Mouse
      • Unigene: 657724 Human
      • Unigene: 33874 Mouse
      • Alternative names

        • CD283 antibody
        • CD283 antigen antibody
        • IIAE2 antibody
        • TLR 3 antibody
        • Tlr3 antibody
        • TLR3_HUMAN antibody
        • Toll Like Receptor 3 antibody
        • Toll-like receptor 3 antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)
        Flow Cytometry - Biotin Anti-TLR3 antibody [TLR3.7] (ab17264)

        Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab17264 at 10 μg/ml.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab17264? Please let us know so that we can cite the reference in this datasheet.

      ab17264 has been referenced in 2 publications.

      • Matsumoto M  et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . PubMed: 12960343
      • Matsumoto M  et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). PubMed: 12054664

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Have you tested the endotoxin level in your anti-TLR3 antibody solution, both biotinylated and unbiotinylated?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 05 2013

      Answer

      I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

      Read More

      Elena Valued Customer

      Abcam Scientific Support

      Answered on Jul 05 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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