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Biotin Anti-TLR4 antibody [MTS510] (ab9105)

Submit a review Q&A (2)References (2)

Key features and details

  • Biotin Rat monoclonal [MTS510] to TLR4
  • Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-TLR4 antibody [MTS510]
    See all TLR4 primary antibodies

  • Description

    Biotin Rat monoclonal [MTS510] to TLR4

  • Host species

    Rat

  • Conjugation

    Biotin

  • Specificity

    Only detects murine TLR-4 when complexed to MD-2. This antibody has been found to coprecipitate MD-2 (30kDa) with TLR4 (100kDa).

  • Tested applications

    Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to TLR4. Mouse Ba/F3 cells expressing mouse TLR4

  • General notes


    Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.

Target

  • Function

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

  • Tissue specificity

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

  • Involvement in disease

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • Domain

    The TIR domain mediates interaction with NOX4.

  • Post-translational
    modifications

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ARMD10 antibody
      • CD284 antibody
      • CD284 antigen antibody
      • Homolog of Drosophila toll antibody
      • hToll antibody
      • TLR 4 antibody
      • TLR4 antibody
      • TLR4_HUMAN antibody
      • TOLL antibody
      • Toll like receptor 4 antibody
      • Toll-like receptor 4 antibody
      see all

    References (2)

    Publishing research using ab9105? Please let us know so that we can cite the reference in this datasheet.

    ab9105 has been referenced in 2 publications.

    • Chakraborty D  et al. Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner. Front Immunol 8:1493 (2017). PubMed: 29180999
    • Daubeuf B  et al. TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 179:6107-14 (2007). Flow Cyt ; Mouse . PubMed: 17947685

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Has this antibody been tested to determine whether it can block LPS induced upregulation of co-stimulatory molecules (e.g cd86)

    Read More
    Answer

    Thank you for your enquiry. ab9105 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to co precipitate MD-2 (30kDa) with TLR4 (100 kDa). The blocking activity of this antibody has been described in the following article: Akashi, S et al; Cutting edge: Cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J Immunol 2000, 164: 3471 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Could you tell me if your antibody against TRL4/MD2 complex, TLR4 antibody [MTS510], could bind to MD-2 alone?

    Read More
    Answer

    Thank you for your enquiry. The antibody recognizes the mouse TLR4/MD2 complex and is not useful for detection of TLR4 or MD2 alone.

    Read More

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