Biotin Anti-TLR9 antibody [5G5] (ab17236)

Reviews (1)Q&A (3)References (4)

Key features and details

  • Biotin Mouse monoclonal [5G5] to TLR9
  • Reacts with: Mouse, Dog, Human
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-TLR9 antibody [5G5]
    See all TLR9 primary antibodies

  • Description

    Biotin Mouse monoclonal [5G5] to TLR9

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Mouse, Dog, Human

  • Immunogen

    Purified fusion protein of the extracellular domain of human TLR9 (AA 1-815) and human IgGFc

  • Positive control

    • RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    References (4)

    Publishing research using ab17236? Please let us know so that we can cite the reference in this datasheet.

    ab17236 has been referenced in 4 publications.

    • Liu FY  et al. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis. Cell Death Dis 10:480 (2019). PubMed: 31209243
    • Chen GY  et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . PubMed: 22704844
    • Chen GY  et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . PubMed: 19656899
    • Tomchuck SL  et al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt ; Human . PubMed: 17916800

    Customer reviews and Q&As

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    Western blot abreview for Anti-TLR9 antibody [5G5] (Biotin)

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    TLR9 knockout
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Abcam user community

    Verified customer

    Submitted Aug 12 2015

    Question

    dear sir,
    I need to buy an anti TLR9 antibody for immunofluorescence staining of murine cells.
    I've read that you have an anti mouse TLR9 in your catalogue (clone 5G5).
    Is possible to have some reference in which this antibody was used, or a fluorescence microscopy image obtained using this antibody, that validate the use of this antibody for immunofluorescence in mice?
    Thanks in advance

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share it on the Website.

    We also encourage our antibody’s users to send us the images obtained with the antibodies and earn Abpoints in compensation: https://www.abcam.com/abpoints.

    However, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Further to our telephone conversation the other day about the use of the biotynlated TLR9 antibody you asked me to confirm the concentration of antibody we were using in our staining procedure. The stock antibody is at a concentration of 0.1mg/ml and I have tried staining 1x 10^6 cells with between 0.1 to 0.5ug/ml of primary antibody. Hope this helps

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    Answer

    Thank you for your e-mail regarding ab17236 - Mouse monoclonal [5G5] to TLR9 (Biotin). We are very sorry to hear the there are still problems with this product and have been discussing this complaint with some colleagues. We looked very carefully the information you provided to us. You mentioned that you tried staining 1x10E6 cells with between 0.1 and 0.5 ug/ml of primary antibody. What is the volume you actually used? We would advise you to use a concentration of 10 ug/ml ( 2 ug/200 ul). It seems that concentration of 0.1 ug/ml are very low. We use the following fixation and permeabilization protocol: - Wash the cells 2x with cold PBS. Temperature control at this point is of vital importance. Note: Cells should be kept on ice any time they are not spinning, and cells should be spun in a temperature controlled centrifuge at 4°C. - Fix the cells with 1% paraformaldehyde for 15 minutes at 4°C. Note: it is crucial to make sure that the cells are resuspended well. - Wash the cells 2x with ice cold PBS. - Permeabilize the cells with 100% ice cold methanol added drop wise while the cells are vortexing. It is crucial to make sure that the cells are resuspended thoroughly. Leave in methanol for 15 minutes. - Wash the cells 2x with cold PBS - Add approximately 1x10E6 cells to each FACS tube and wash with 1 ml of 0.1% saponin diluted in PBS with 2% fetal bovine serum added. - Resuspend cells in 1 ml of 0.1% saponin + 2% FBS and allow to sit for 30 minutes blocking at RT. - Add primary antibody We are not sure why you washed the cells between the permeabilization step and the primary antibody incubation. We would like to advise you to follow the above protocol (if this is not the protocol you normally use). I hope this information will be useful for you, please do not hesitate to contact Abcam if you still have further questions.

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    Question

    May I see a western blot plot for ab17236 anti TLR9 (5G5)?

    Read More
    Answer

    Thank you for your enquiry. This Ab is cited int following paper being used for WB: Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958 Contained within this paper is a Western blot image pertaining to this product. If you need any further assistance then please get back in touch with us.

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