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  1. Link

    biotin-tlr9-antibody-5g5-ab17236.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Biotin Anti-TLR9 antibody [5G5] (ab17236)

  • Datasheet
Reviews (1)Q&A (3)References (5)

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Key features and details

  • Biotin Mouse monoclonal [5G5] to TLR9
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Dog, Human
  • Conjugation: Biotin
  • Isotype: IgG2a

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Overview

  • Product name

    Biotin Anti-TLR9 antibody [5G5]
    See all TLR9 primary antibodies
  • Description

    Biotin Mouse monoclonal [5G5] to TLR9
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human
  • Immunogen

    Recombinant fragment within TLR9. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

  • Positive control

    • RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purification notes

    0.2 µm filtered
  • Clonality

    Monoclonal
  • Clone number

    5G5
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • FITC Anti-TLR9 antibody [5G5] (ab58864)
  • Isotype control

    • Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)
  • Recombinant Protein

    • Recombinant Human TLR9 protein (ab153365)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB (1)
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Target information above from: UniProt accession Q9NR96 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54106 Human
    • Entrez Gene: 81897 Mouse
    • Omim: 605474 Human
    • SwissProt: Q9NR96 Human
    • SwissProt: Q9EQU3 Mouse
    • Unigene: 87968 Human
    • Unigene: 44889 Mouse
    • Alternative names

      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (5)

    Publishing research using ab17236? Please let us know so that we can cite the reference in this datasheet.

    ab17236 has been referenced in 5 publications.

    • Wang CY  et al. TLR9 Binding to Beclin 1 and Mitochondrial SIRT3 by a Sodium-Glucose Co-Transporter 2 Inhibitor Protects the Heart from Doxorubicin Toxicity. Biology (Basel) 9:N/A (2020). PubMed: 33138323
    • Liu FY  et al. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis. Cell Death Dis 10:480 (2019). PubMed: 31209243
    • Chen GY  et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . PubMed: 22704844
    • Chen GY  et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . PubMed: 19656899
    • Tomchuck SL  et al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt ; Human . PubMed: 17916800

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-TLR9 antibody [5G5] (Biotin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    TLR9 knockout
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2015

    Question

    dear sir,
    I need to buy an anti TLR9 antibody for immunofluorescence staining of murine cells.
    I've read that you have an anti mouse TLR9 in your catalogue (clone 5G5).
    Is possible to have some reference in which this antibody was used, or a fluorescence microscopy image obtained using this antibody, that validate the use of this antibody for immunofluorescence in mice?
    Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2012

    Answer

    Thank you for contacting us.

    Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share it on the Website.

    We also encourage our antibody’s users to send us the images obtained with the antibodies and earn Abpoints in compensation: https://www.abcam.com/abpoints.

    However, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2012

    Question

    Further to our telephone conversation the other day about the use of the biotynlated TLR9 antibody you asked me to confirm the concentration of antibody we were using in our staining procedure. The stock antibody is at a concentration of 0.1mg/ml and I have tried staining 1x 10^6 cells with between 0.1 to 0.5ug/ml of primary antibody. Hope this helps

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2006

    Answer

    Thank you for your e-mail regarding ab17236 - Mouse monoclonal [5G5] to TLR9 (Biotin). We are very sorry to hear the there are still problems with this product and have been discussing this complaint with some colleagues. We looked very carefully the information you provided to us. You mentioned that you tried staining 1x10E6 cells with between 0.1 and 0.5 ug/ml of primary antibody. What is the volume you actually used? We would advise you to use a concentration of 10 ug/ml ( 2 ug/200 ul). It seems that concentration of 0.1 ug/ml are very low. We use the following fixation and permeabilization protocol: - Wash the cells 2x with cold PBS. Temperature control at this point is of vital importance. Note: Cells should be kept on ice any time they are not spinning, and cells should be spun in a temperature controlled centrifuge at 4°C. - Fix the cells with 1% paraformaldehyde for 15 minutes at 4°C. Note: it is crucial to make sure that the cells are resuspended well. - Wash the cells 2x with ice cold PBS. - Permeabilize the cells with 100% ice cold methanol added drop wise while the cells are vortexing. It is crucial to make sure that the cells are resuspended thoroughly. Leave in methanol for 15 minutes. - Wash the cells 2x with cold PBS - Add approximately 1x10E6 cells to each FACS tube and wash with 1 ml of 0.1% saponin diluted in PBS with 2% fetal bovine serum added. - Resuspend cells in 1 ml of 0.1% saponin + 2% FBS and allow to sit for 30 minutes blocking at RT. - Add primary antibody We are not sure why you washed the cells between the permeabilization step and the primary antibody incubation. We would like to advise you to follow the above protocol (if this is not the protocol you normally use). I hope this information will be useful for you, please do not hesitate to contact Abcam if you still have further questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 03 2006

    Question

    May I see a western blot plot for ab17236 anti TLR9 (5G5)?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2005

    Answer

    Thank you for your enquiry. This Ab is cited int following paper being used for WB: Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958 Contained within this paper is a Western blot image pertaining to this product. If you need any further assistance then please get back in touch with us.

    Read More

    Abcam Scientific Support

    Answered on Mar 03 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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