Biotin Anti-Toxoplasma gondii antibody (ab69251)

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Key features and details

  • Biotin Goat polyclonal to Toxoplasma gondii
  • Suitable for: ELISA
  • Reacts with: Toxoplasma gondii
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-Toxoplasma gondii antibody
    See all Toxoplasma gondii primary antibodies

  • Description

    Biotin Goat polyclonal to Toxoplasma gondii

  • Host species

    Goat

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Toxoplasma gondii

  • Immunogen

    RH Strain of Toxoplasma gondii.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Target

  • Relevance

    Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

  • Database links

    • Alternative names

      • T. gondii antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab69251? Please let us know so that we can cite the reference in this datasheet.

    ab69251 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I wonder if I can order the item which has Cat# ab69250 I did not found it on the abcam company online page. Fore your information I found the closet product to the Cat# ab69250 is Cat# ab69251 do you advice me to order it? if yes which type of secondary antibody I can use to detect it

    please suggest to me which antibody I can use instead and which item can I use to detect it

    Read More
    Answer

    Thank you for contacting us.

    If you are looking for an antibody to detect Toxoplasma gondii in frozen tissue sections, then the best choice is probably ab15170 or ab23507. Even though neither have been tested on sections of frozen tissue, the IHC data for detection in paraffin-embedded tissue is very clean and specific, for ab15170, and detection in frozen tissue is generally easier than detection in formalin-fixed paraffin-embedded tissue. The alternative, goat polyclonal ab23507, has been published twice.

    Our guarantee does not apply to IHC of frozen tissue using either of these antibodies, but I can offer a testing discount for either, if you are interested. For details, please see the web page at the following URL:

    www.abcam.com/abtrial

    For detection of ab15170, you will need an anti-rabbit IgG secondary antibody conjugated to an enzyme such as HRP, or a fluorochrome such as FITC. For an HRP conjugate, we recommend ab6802:

    Click here (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

    For the goat polyclonal ab23507, we recommend the HRP conjugate ab6741:

    Click here (or use the following: https://www.abcam.com/Rabbit-Goat-IgG-HL-HRP-ab6741.html).

    For fluorochrome conjugates, please reply and I will help.

    Read More

    Question

    1- I will select a Primary antibody [(Anti-Toxoplasma gondii antibody (Biotin) (ab69251)], I wonder if the secondary antibody that I most to choose [(Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)] is work with the primary antibody [(Anti-Toxoplasma gondii antibody (Biotin) (ab69251)].

    2- Would you please advise me which kind of buffer and the dilution factor I can use to dilute the primary as well as the secondary antibodies for ELISA test.

    Read More
    Answer

    Thank you for contacting us.

    The secondary antibody ab6741 will probably recognize the biotinylated antibody ab69251 but they have not been tested together. You may want to consider using the streptavidin-HRP conjugate ab7403 instead of a secondary antibody. Or, you may want to order a different primary antibody, without biotin, such as the HRP-conjugated ab21182:

    Click here (or use the following: https://www.abcam.com/Toxoplasma-gondii-antibody-HRP-ab21182.html).

    Click here (or use the following: https://www.abcam.com/Streptavidin-HRP-ab7403.html).

    The Toxoplasma gondii antibody ab69251 should be used at a dilution of 1/1000 but you may need to optimize the dilution factor depending on your results. I recommend diluting the antibody in Tris-buffered saline, pH 7.4 for detection. The secondary ab6741 can be applied very dilute at 1/75,000 but again, this may need to optimized since these two antibodies have not been used together before.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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