Biotin Anti-TRAIL antibody [N2B2] (ab95531)
![Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)](https://www.abcam.com/ps/products/95/ab95531/Images/ab95531-109816-anti-trail-antibody-n2b2-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)")
Key features and details
- Biotin Rat monoclonal [N2B2] to TRAIL
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TRAIL antibody [N2B2]
See all TRAIL primary antibodies -
Description
Biotin Rat monoclonal [N2B2] to TRAIL -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse TRAIL-transfected 2PK-3 cells.
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Positive control
- Mouse TRAIL transfected cells
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General notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells. -
Clonality
Monoclonal -
Clone number
N2B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.125-0.25µg for 105-8 cells. in a final volume of 100 µL.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22035 Mouse
- SwissProt: P50592 Mouse
- Unigene: 1062 Mouse
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
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Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)ab95531, at 0.125 µg, staining of TRAIL transfected cells (filled) or with Rat IgG2a Isotype Control (open) followed by Streptavidin PE by Flow Cytometry. Total viable cells were used for analysis.
Datasheets and documents
References (2)
ab95531 has been referenced in 2 publications.
- Hayakawa Y et al. NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy. J Immunol 172:123-9 (2004). PubMed: 14688317
- Kayagaki N et al. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol 163:1906-13 (1999). Flow Cyt ; Mouse . PubMed: 10438925
