Biotin Anti-TRAIL antibody [N2B2] (ab95531)
![Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)](https://www.abcam.com/ps/products/95/ab95531/Images/ab95531-109816-anti-trail-antibody-n2b2-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)")
Key features and details
- Biotin Rat monoclonal [N2B2] to TRAIL
- Suitable for: Flow Cyt
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TRAIL antibody [N2B2]
See all TRAIL primary antibodies -
Description
Biotin Rat monoclonal [N2B2] to TRAIL -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Immunogen
Mouse TRAIL-transfected 2PK-3 cells.
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Positive control
- Mouse TRAIL transfected cells
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General notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells. -
Clonality
Monoclonal -
Clone number
N2B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.125-0.25µg for 105-8 cells.
in a final volume of 100 µL.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 0.125-0.25µg for 105-8 cells. in a final volume of 100 µL.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
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Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)ab95531, at 0.125 µg, staining of TRAIL transfected cells (filled) or with Rat IgG2a Isotype Control (open) followed by Streptavidin PE by Flow Cytometry. Total viable cells were used for analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95531 has been referenced in 2 publications.
- Hayakawa Y et al. NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy. J Immunol 172:123-9 (2004). PubMed: 14688317
- Kayagaki N et al. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol 163:1906-13 (1999). Flow Cyt ; Mouse . PubMed: 10438925