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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Biotin Anti-TRAIL antibody [N2B2] (ab95531)

  • Datasheet
  • SDS
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Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)

    Key features and details

    • Biotin Rat monoclonal [N2B2] to TRAIL
    • Suitable for: Flow Cyt
    • Conjugation: Biotin
    • Isotype: IgG2a

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    Overview

    • Product name

      Biotin Anti-TRAIL antibody [N2B2]
      See all TRAIL primary antibodies
    • Description

      Biotin Rat monoclonal [N2B2] to TRAIL
    • Host species

      Rat
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Immunogen

      Mouse TRAIL-transfected 2PK-3 cells.

    • Positive control

      • Mouse TRAIL transfected cells
    • General notes


      ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.87% Sodium chloride, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Primary antibody notes

      ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.
    • Clonality

      Monoclonal
    • Clone number

      N2B2
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Death receptors & ligands
      • TRAIL
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Receptors
      • Death receptors & ligands
      • TRAIL

    Associated products

    • Alternative Versions

      • PE Anti-TRAIL antibody [N2B2] (ab95532)
    • Isotype control

      • Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)
    • Recombinant Protein

      • Recombinant human TRAIL protein (ab9960)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab95531 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 0.125-0.25µg for 105-8 cells.

    in a final volume of 100 µL.

     

     

     

    ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 0.125-0.25µg for 105-8 cells.

    in a final volume of 100 µL.

     

     

     

    ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
    • Tissue specificity

      Widespread; most predominant in spleen, lung and prostate.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P50591 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    Images

    • Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)
      Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (ab95531)
      ab95531, at 0.125 µg, staining of TRAIL transfected cells (filled) or with Rat IgG2a Isotype Control (open) followed by Streptavidin PE by Flow Cytometry. Total viable cells were used for analysis.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab95531? Please let us know so that we can cite the reference in this datasheet.

    ab95531 has been referenced in 2 publications.

    • Hayakawa Y  et al. NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy. J Immunol 172:123-9 (2004). PubMed: 14688317
    • Kayagaki N  et al. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol 163:1906-13 (1999). Flow Cyt ; Mouse . PubMed: 10438925

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