Biotin Anti-TRAIL antibody [N2B2] (ab95531)
Key features and details
- Biotin Rat monoclonal [N2B2] to TRAIL
- Suitable for: Flow Cyt
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TRAIL antibody [N2B2]
See all TRAIL primary antibodies -
Description
Biotin Rat monoclonal [N2B2] to TRAIL -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Immunogen
Mouse TRAIL-transfected 2PK-3 cells.
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Positive control
- Mouse TRAIL transfected cells
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General notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells. -
Clonality
Monoclonal -
Clone number
N2B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.125-0.25µg for 105-8 cells.
in a final volume of 100 µL.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.125-0.25µg for 105-8 cells. in a final volume of 100 µL.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95531 has been referenced in 2 publications.
- Hayakawa Y et al. NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy. J Immunol 172:123-9 (2004). PubMed: 14688317
- Kayagaki N et al. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol 163:1906-13 (1999). Flow Cyt ; Mouse . PubMed: 10438925