Key features and details
- Biotin Rat monoclonal [R17217.1.4] to Transferrin Receptor
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-Transferrin Receptor antibody [R17217.1.4]
See all Transferrin Receptor primary antibodies
DescriptionBiotin Rat monoclonal [R17217.1.4] to Transferrin Receptor
SpecificityThe clone number has been updated from (1.BB.713) to (R17217.1.4) both clone numbers name the same antibody clone.
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse Transferrin Receptor. DMSO induced Friend erythroleukemia 745.6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notespurified by affinity chromatography
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
In vivo and vitro inhibition of cell proliferation: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
ab63333 has not yet been referenced specifically in any publications.