Biotin Anti-Transferrin Receptor antibody [R17217.1.4] (ab63333)
Key features and details
- Biotin Rat monoclonal [R17217.1.4] to Transferrin Receptor
- Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
-
Product name
Biotin Anti-Transferrin Receptor antibody [R17217.1.4]
See all Transferrin Receptor primary antibodies -
Description
Biotin Rat monoclonal [R17217.1.4] to Transferrin Receptor -
Host species
Rat -
Conjugation
Biotin -
Specificity
The clone number has been updated from (1.BB.713) to (R17217.1.4) both clone numbers name the same antibody clone. -
Tested applications
Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Transferrin Receptor. DMSO induced Friend erythroleukemia 745.6
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
purified by affinity chromatography -
Clonality
Monoclonal -
Clone number
R17217.1.4 -
Isotype
IgG2a -
Research areas
Associated products
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use 1µg for 106 cells.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 22042 Mouse
- SwissProt: Q62351 Mouse
-
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab63333 has not yet been referenced specifically in any publications.