Biotin Anti-TRAP/CD40L antibody [24-31] (ab134407)
![Flow Cytometry - Biotin Anti-TRAP/CD40L antibody [24-31] (ab134407)](https://www.abcam.com/ps/products/134/ab134407/Images/ab134407-148740-anti-trap-cd40l-antibody-24-31-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-TRAP/CD40L antibody [24-31] (ab134407)")
Key features and details
- Biotin Mouse monoclonal [24-31] to TRAP/CD40L
- Suitable for: Flow Cyt
- Reacts with: Human, Cynomolgus monkey, Rhesus monkey
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-TRAP/CD40L antibody [24-31]
See all TRAP/CD40L primary antibodies -
Description
Biotin Mouse monoclonal [24-31] to TRAP/CD40L -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Cynomolgus monkey, Rhesus monkey -
Immunogen
Fusion protein corresponding to TRAP/CD40L.
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Positive control
- TPA+ ionomycin-stimulated Human PBMCs
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General notes
Store undiluted.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
24-31 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- FITC Anti-TRAP/CD40L antibody [24-31] (ab134404)
- PE Anti-TRAP/CD40L antibody [24-31] (ab134405)
- APC Anti-TRAP/CD40L antibody [24-31] (ab134406)
- Anti-TRAP/CD40L antibody [24-31] - Low endotoxin, Azide free (ab234178)
- PE Anti-TRAP/CD40L antibody [24-31] (ab234220)
- FITC Anti-TRAP/CD40L antibody [24-31] (ab234271)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5µg for 106 cells. Use 100 µl test volume. |
Target
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Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
Tissue specificity
Specifically expressed on activated CD4+ T-lymphocytes. -
Involvement in disease
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 102138630 Cynomolgus monkey
- Entrez Gene: 959 Human
- Omim: 300386 Human
- SwissProt: P29965 Human
- Unigene: 592244 Human
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Alternative names
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
Images
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Flow Cytometry - Biotin Anti-TRAP/CD40L antibody [24-31] (ab134407)
Flow cytometric analysis of TRAP/CD40L in TPA and ionomycin-stimulated Human PBMCs stained with ab134407 at 0.5 µg per 10 6 cells (in 100 µl volume), followed by Sav-PE. PBMCs were stimulated for 5 hours prior to analysis.
Datasheets and documents
References (2)
ab134407 has been referenced in 2 publications.
- Matus-Nicodemos R et al. Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71 (2011). Flow Cyt ; Human . PubMed: 21242519
- Kuchen S et al. Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol 179:5886-96 (2007). Flow Cyt ; Human . PubMed: 17947662
