Biotin Anti-V5 tag antibody [SV5-Pk1] (ab27672)

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Key features and details

  • Biotin Mouse monoclonal [SV5-Pk1] to V5 tag
  • Suitable for: IHC-Fr, ELISA, WB
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-V5 tag antibody [SV5-Pk1]
    See all V5 tag primary antibodies

  • Description

    Biotin Mouse monoclonal [SV5-Pk1] to V5 tag

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-Fr, ELISA, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Mice were infected with the paramyxovirus SV5, Simian-Virus 5

  • Epitope

    Ab27672 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    This antibody was purified from tissue culture supernatant by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    SV5-Pk1

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
Notes
IHC-Fr
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.

Target

  • Relevance

    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

  • Alternative names

    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References (0)

Publishing research using ab27672? Please let us know so that we can cite the reference in this datasheet.

ab27672 has not yet been referenced specifically in any publications.

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