Biotin Anti-Vaccinia Virus antibody (ab21039)
Key features and details
- Biotin Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA
- Reacts with: Vaccinia virus
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Vaccinia Virus antibody
See all Vaccinia Virus primary antibodies -
Description
Biotin Rabbit polyclonal to Vaccinia Virus -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Vaccinia virus -
Immunogen
Tissue, cells or virus corresponding to Vaccinia Virus. Lister Strain (mixture of virions and infected cell polypeptides).
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General notes
The antibody is covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent dilution.
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| Notes |
|---|
|
ELISA
Use at an assay dependent dilution. |
Target
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Relevance
Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process. -
Alternative names
- A27L antibody
- Orthopoxvirus antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab21039 has been referenced in 2 publications.
- Kim JS et al. The resveratrol analogue, HS-1793, enhances the effects of radiation therapy through the induction of anti-tumor immunity in mammary tumor growth. Int J Oncol 56:1405-1416 (2020). PubMed: 32236622
- Ylösmäki E et al. Personalized Cancer Vaccine Platform for Clinically Relevant Oncolytic Enveloped Viruses. Mol Ther 26:2315-2325 (2018). PubMed: 30005865