Biotinylated Goat anti-Mouse & Rabbit IgG (H+L) (Ready To Use) (ab64257)
Overview
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Product name
Biotinylated Goat anti-Mouse & Rabbit IgG (H+L) (Ready To Use)
See all IgG reagents -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Staining protocol
- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add enough drops of an appropriate hydrogen peroxide block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply an appropriate protein block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse & Rabbit IgG(H+L) solution (ab64257) and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Reagent notes
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
Cellular localization
Secreted
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent dilution.
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Notes |
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IHC-P
Use at an assay dependent dilution. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab64257 has been referenced in 10 publications.
- Smith SM et al. Paired associates learning is disrupted after unilateral parietal lobe controlled cortical impact in rats: A trial-by-trial behavioral analysis. Behav Brain Res 437:114106 (2023). PubMed: 36089100
- Mota RF et al. RANKL Impairs the TLR4 Pathway by Increasing TRAF6 and RANK Interaction in Macrophages. Biomed Res Int 2022:7740079 (2022). PubMed: 35463988
- Zeidi M et al. Increased CD69+CCR7+ circulating activated T cells and STAT3 expression in cutaneous lupus erythematosus patients recalcitrant to antimalarials. Lupus 31:472-481 (2022). PubMed: 35258358
- Zeidi M et al. Increased MxA protein expression and dendritic cells in spongiotic dermatitis differentiates dermatomyositis from eczema in a single-center case-control study. J Cutan Pathol 48:364-373 (2021). PubMed: 32954523
- Chen KL et al. Myeloid Dendritic Cells Are Major Producers of IFN-β in Dermatomyositis and May Contribute to Hydroxychloroquine Refractoriness. J Invest Dermatol 141:1906-1914.e2 (2021). PubMed: 33675790
- Diaz-Lucena D et al. TREM2 expression in the brain and biological fluids in prion diseases. Acta Neuropathol 141:841-859 (2021). PubMed: 33881612
- Zhao QH et al. Matrix metalloproteinase-13, NF-?B p65 and interleukin-1ß are associated with the severity of knee osteoarthritis. Exp Ther Med 19:3620-3626 (2020). PubMed: 32346426
- Li R et al. Tree shrew as a new animal model to study the pathogenesis of avian influenza (H9N2) virus infection. Emerg Microbes Infect 7:166 (2018). PubMed: 30301950
- Xu H et al. Inhibitor ?Ba protein therapy alleviates severe pneumonia through inhibition of nuclear factor ?B. Exp Ther Med 13:1398-1402 (2017). PubMed: 28413484
- Fei B et al. Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages. Oncol Lett 11:2271-2277 (2016). PubMed: 26998161