Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) (ab201796)

Overview

  • Product name
    Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B)
  • Product overview

    Biotinylation Kit / Biotin Conjugation Kit ab201796 provides an easy-to-use, one step procedure that allows researchers to directly label proteins, peptides and other biomolecules containing primary amines with biotin with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.


    The Type B Biotinylation Kit / Biotin Conjugation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).


    Use the Type A Biotinylation Kit / Biotin Conjugation Kit ab201795 to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.


    Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    30 μg containing 3 reactions (each for 10 - 20 μg)

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab201796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

Protocols

References

This product has been referenced in:
  • Lino Cardenas CL  et al. An HDAC9-MALAT1-BRG1 complex mediates smooth muscle dysfunction in thoracic aortic aneurysm. Nat Commun 9:1009 (2018). Read more (PubMed: 29520069) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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