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Biotinylation Kit / Biotin Conjugation Kit ab201796 provides an easy-to-use, one step procedure that allows researchers to directly label proteins, peptides and other biomolecules containing primary amines with biotin with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
The Type B Biotinylation Kit / Biotin Conjugation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).
Use the Type A Biotinylation Kit / Biotin Conjugation Kit ab201795 to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
30 μg containing 3 reactions (each for 10 - 20 μg)
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||30 µg||300 µg||1 mg|
|Biotin (Type B) Conjugation Mix||3 vials||3 vials||1 vial|
|Biotin (Type B) Modifier Reagent||1 vial||1 vial||1 vial|
|Biotin (Type B) Quencher Reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab201796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"