Overview

  • Product name

    Anti-BirA antibody [6C4c7]
  • Description

    Mouse monoclonal [6C4c7] to BirA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
    Database link: P06709

  • Positive control

    • WB: BirA-transfected HEK293T whole cell lysates (induced).
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6C4c7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.

Target

  • Relevance

    BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
  • Database links

    • Alternative names

      • Bifunctional protein birA antibody
      • Bio R antibody
      • BioR antibody
      • Biotin [acetyl CoA carboxylase] synthetase antibody
      • Biotin ligase antibody
      • Biotin operon repressor antibody
      • Biotin protein ligase antibody
      • Bir A antibody
      • BirA bifunctional protein antibody
      • Dhb B antibody
      • dhbB antibody
      see all

    Images

    • All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HEK293T whole cell lysate
      Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
      Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

      Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.

    References

    ab232732 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab232732.
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