Product nameAnti-BirA antibody [6C4c7]
DescriptionMouse monoclonal [6C4c7] to BirA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Escherichia coli
Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
Database link: P06709
- WB: BirA-transfected HEK293T whole cell lysates (induced).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab232732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
RelevanceBirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
- Bifunctional protein birA antibody
- Bio R antibody
- BioR antibody
All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
ab232732 has not yet been referenced specifically in any publications.