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Rabbit polyclonal to BIRC6
Predicted to work with:
Mouse, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to BIRC6 (C terminal).
Database link: Q9NR09
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/5000 - 1/15000. Predicted molecular weight: 528 kDa.
Use at 1-4 µg/mg of lysate.
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protects cells from undergoing apoptosis.
Expressed in brain cancer cells.
In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.
Contains 1 BIR repeat.
Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis.
Information by UniProt
Apollon protein antibody
Baculoviral IAP repeat containing 6 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6 antibody (ab19609)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BIRC6 with ab19609 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - Anti-BIRC6 antibody (ab19609)
All lanes :
Anti-BIRC6 antibody (ab19609) at 1/10000 dilution
Lane 1 :
HeLa whole cell lysate at 20 µg
Lane 2 :
HeLa whole cell lysate at 50 µg
Lane 3 :
HeLa S100 extract at 20 µg
Lane 4 :
HeLa S100 extract at 50 µg
Predicted band size:
Detection of Human BIRC6 by western blot.
Lane 1: 20 g of HeLa whole cell lysate. µ Lane 2: 50 g of HeLa whole cell lysate. µ Lane 3: 20 g of HeLa S100 extract. µ Lane 4: 50 g of HeLa S100 extract. µ
Affinity purified anti-BIRC antibody (ab19609) was used at a 1/10,000 dilution.
Immunoprecipitation - Anti-BIRC6 antibody (ab19609)
Detection of human BIRC6 by Immunoprecipitation. Affinity purified ab19609 used at 2mcg/mg lysate (whole cell lysate (10mg) from HeLa cells. The immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.
Immunocytochemistry/ Immunofluorescence - Anti-BIRC6 antibody (ab19609)
ICC/IF image of ab19609 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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