Overview

  • Product name

    Anti-BIRC6/APOLLON antibody
    See all BIRC6/APOLLON primary antibodies
  • Description

    Rabbit polyclonal to BIRC6/APOLLON
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to BIRC6/APOLLON (C terminal).
    Database link: Q9NR09

  • General notes

     This product was previously labelled as BIRC6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab19609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Predicted molecular weight: 528 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Protects cells from undergoing apoptosis.
  • Tissue specificity

    Expressed in brain cancer cells.
  • Sequence similarities

    In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 BIR repeat.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apollon antibody
    • Apollon protein antibody
    • Baculoviral IAP repeat containing 6 antibody
    • Baculoviral IAP repeat containing protein 6 antibody
    • Baculoviral IAP repeat-containing protein 6 antibody
    • BIR repeat containing ubiquitin conjugating enzyme antibody
    • BIRC 6 antibody
    • BIRC6 antibody
    • BIRC6_HUMAN antibody
    • BRUCE antibody
    • FLJ13726 antibody
    • FLJ13786 antibody
    • KIAA1289 antibody
    • Ubiquitin conjugating BIR domain enzyme apollon antibody
    • Ubiquitin-conjugating BIR domain enzyme apollon antibody
    see all

Images

  • All lanes : Anti-BIRC6/APOLLON antibody (ab19609) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 20 µg
    Lane 2 : HeLa whole cell lysate at 50 µg
    Lane 3 : HeLa S100 extract at 20 µg
    Lane 4 : HeLa S100 extract at 50 µg

    Predicted band size: 528 kDa



    Detection of Human BIRC6/APOLLON by western blot.
    Lane 1:  20 µg of HeLa whole cell lysate.
    Lane 2:  50 µg of HeLa whole cell lysate.
    Lane 3:  20 µg of HeLa S100 extract.
    Lane 4:  50 µg of HeLa S100 extract.

    Affinity purified anti-BIRC antibody (ab19609) was used at a 1/10,000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BIRC6/APOLLON with ab19609 at 1/5000 (0.2µg/ml). Detection: DAB.

  • Detection of human BIRC6/APOLLON by Immunoprecipitation. Affinity purified ab19609 used at 2mcg/mg lysate (whole cell lysate (10mg) from HeLa cells. The immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6/APOLLON was confirmed by mass spectrometry.

  • ICC/IF image of ab19609 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Park JS  et al. Alpha-lipoic acid attenuates p-cresyl sulfate-induced renal tubular injury through suppression of apoptosis and autophagy in human proximal tubular epithelial cells. Biomed Pharmacother 112:108679 (2019). Read more (PubMed: 30798133) »
  • Okumu DO  et al. BIRC6 mediates imatinib resistance independently of Mcl-1. PLoS One 12:e0177871 (2017). WB ; Human . Read more (PubMed: 28520795) »
See all 8 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Patient fibroblasts)
Gel Running Conditions
Reduced Denaturing (6 %)
Loading amount
15 µg
Specification
Patient fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 30 2017

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