Product nameBis-Tris propane, buffer
DescriptionBuffer with a wide buffering range.
- bis tris propane
Buffer that is useful in the pH 6.3-9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Wenner JR & Bloomfield VA Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem 268:201-12 (1999). Read more (PubMed: 10075809) »
- Eckert KA & Kunkel TA DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl 1:17-24 (1991). Read more (PubMed: 1842916) »