Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain (ab145596)

Overview

  • Product name

    Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain
  • Description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent
  • Alternative names

    • Hoechst 33258
  • Biological description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33342 (ab145596) analog.

  • Purity

    > 98%
  • CAS Number

    23491-45-4
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    350nm
  • Emission

    461nm
  • Chemical name

    4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride
  • Molecular weight

    533.88
  • Molecular formula

    C25H24N6O.3HCl
  • PubChem identifier

    5360743
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Zurek-Biesiada D  et al. UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms. Cytometry A 83:441-51 (2013). Read more (PubMed: 23418106) »
  • Bazhulina NP  et al. Binding of Hoechst 33258 and its derivatives to DNA. J Biomol Struct Dyn 26:701-18 (2009). Read more (PubMed: 19385699) »
  • Khan QA & Pilch DS Topoisomerase I-mediated DNA cleavage induced by the minor groove-directed binding of bibenzimidazoles to a distal site. J Mol Biol 365:561-9 (2007). Read more (PubMed: 17095016) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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