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Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (ab145597)

  • Datasheet
  • SDS
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Key features and details

  • Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent.
  • CAS Number: 23491-52-3
  • Purity: > 98%
  • Soluble in water to 75 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain
  • Description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent.
  • Alternative names

    • Hoechst 33342
  • Biological description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

  • Purity

    > 98%
  • CAS Number

    23491-52-3
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    360nm
  • Emission

    600nm
  • Chemical name

    2-(4-Ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole trihydrochloride
  • Molecular weight

    561.93
  • Molecular formula

    C27H28N6O.3HCl
  • PubChem identifier

    16760503
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 75 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl
  • Source

    Synthetic

  • Research areas

    • Biochemicals
    • Product Range
    • New
    • Biochemicals
    • Product Range
    • Just Add Water
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Chemical Type
    • Fluorescent tools
    • Biochemicals
    • Pharmacology
    • Signaling
    • DNA, RNA & protein synthesis
    • Topoisomerase

Associated products

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab145597? Please let us know so that we can cite the reference in this datasheet.

    ab145597 has been referenced in 5 publications.

    • Sudaryatma PE  et al. Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells. Vet Microbiol 246:108748 (2020). PubMed: 32605748
    • Ming-Yan Y  et al. Liraglutide inhibits the apoptosis of human nucleus pulposus cells induced by high glucose through PI3K/Akt/caspase-3 signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31383790
    • Pazinato FM  et al. Immunolocalization of Leptin and its Receptor (ObR-b) in Equine Placenta at Term and Plasma Level Measurement in the Late Gestation. J Equine Vet Sci 78:1-5 (2019). PubMed: 31203970
    • Sudaryatma PE  et al. Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria. Vet Microbiol 235:80-85 (2019). PubMed: 31282382
    • Tian NX  et al. KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats. J Neurosci 38:1756-1773 (2018). PubMed: 29335353

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