Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (ab145597)

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Overview

  • Product name

    Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

  • Description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent.

  • Alternative names

    • Hoechst 33342

  • Biological description

    Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

  • Purity

    > 98%

  • CAS Number

    23491-52-3

  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    360nm

  • Emission

    600nm

  • Chemical name

    2-(4-Ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole trihydrochloride

  • Molecular weight

    561.93

  • Molecular formula

    C27H28N6O.3HCl

  • PubChem identifier

    16760503

  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

  • Solubility overview

    Soluble in water to 75 mM

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl

  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Khan QA & Pilch DS Topoisomerase I-mediated DNA cleavage induced by the minor groove-directed binding of bibenzimidazoles to a distal site. J Mol Biol 365:561-9 (2007). Read more (PubMed: 17095016) »
  • Mann SL  et al. Comparative genomic hybridization analysis of sperm DNA apoptosis after exposure to heat shock. J Assist Reprod Genet 19:195-200 (2002). Read more (PubMed: 12036087) »
  • Chen AY  et al. DNA minor groove-binding ligands: a different class of mammalian DNA topoisomerase I inhibitors. Proc Natl Acad Sci U S A 90:8131-5 (1993). Read more (PubMed: 7690143) »

Publishing research using ab145597? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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