Bisulfite Conversion Kit - Whole Cell (ab117126)


  • Product name
    Bisulfite Conversion Kit - Whole Cell
    See all DNA modification kits
  • Sample type
    Saliva, Urine, Tissue, Adherent cells, Suspension cells
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.

     Bisulfite Modification Kit - Whole Cell (ab117126) allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. ab117126 is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.

    Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.

    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

  • Notes






  • MCF-7 cells were modified using ab117126. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of ß-actin was used.



ab117126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

After bisulfite the DNA will be in ssDNA form and can not be directly used for ligation with illumina's adaptor. We recommend to use ab117127 (enhancer program) to bisulfite modifying DNA after ligation with Illumina's adaptor as indicated by Illumina ...

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Thank you very for having patience!
If use extracted DNA, the procedure can be processed starting from step 3 with slight modification:

3. Add 1 ml of EBM5 to 1 vial of EBM4, followed by adding 60 µl of EBM6 to create the EBM4/EBM5/EBM6 so...

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The kit can be used with tissue as low as 1 microgram and with paraffin embedded tissue sections. The procedure is given in attached booklet;

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Thank you for your patience regarding this shipment of the digestion buffer for ab117126. I am very sorry for the delay. I have issued a free of charge replacement.You should receive this tomorrow.

Please note that this free of charge replace...

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