Overview

  • Product name
    Bisulfite-Seq High Sensitivity Kit (For Illumina®)
  • Sensitivity
    < 0.5 ng
  • Assay time
    8h 00m
  • Product overview

    ab185907 is designed to carry out bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit. The DNA library is constructed directly from bisulfite-converted DNA generated from a small amount of input DNA (500 pg to 500 ng). Intended applications include whole genome bisulfite sequencing, oxidative bisulfite sequencing, reduced representative bisulfite sequencing, and various other bisulfite-next generation sequencing techniques. The optimized protocol and components of the kit allow the DNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using sub-nanogram quantities of bisulfite converted DNA.

     

  • Notes

    DNA methylation occurs by the covalent addition of a methyl group (CH3) at the 5-carbon of the cytosine ring, resulting in 5-methylcytosine (5-mC). DNA methylation is essential in regulating gene expression in nearly all biological processes including development, growth, and differentiation. Alterations in DNA methylation have been demonstrated to cause a change in gene expression. Genome-wide analysis of DNA methylation could provide valuable information for discovering epigenetic markers used for disease diagnosis, and potential targets used for therapeutics. Bisulfite sequencing via next-generation sequencing technologies allow for high volume, lower cost output of DNA sequence data towards a better understanding of DNA methylation.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 24 tests 12 tests
    10X dA-Tailing Buffer 1 x 80µl 1 x 40µl
    10X End Repair Buffer 1 x 80µl 1 x 40µl
    2X HiFi PCR Master Mix 1 x 320µl 1 x 160µl
    2X Ligation Buffer 1 x 500µl 1 x 250µl
    5X Conversion Buffer 1 x 100µl 1 x 50µl
    Adaptors (50 μM) 1 x 30µl 1 x 15µl
    Conversion Enzyme Mix 1 x 30µl 1 x 15µl
    Conversion Primer 1 x 52µl 1 x 26µl
    Desulphonation Solution 1 x 140µl 1 x 70µl
    DNA Binding Solution 1 x 12ml 1 x 6ml
    Elution Buffer 1 x 2ml 1 x 1ml
    Elution Solution 1 x 1ml 1 x 0.5ml
    End Repair Enzyme Mix 1 x 50µl 1 x 25µl
    F-Collection Tube 1 x 30 units 1 x 15 units
    F-Spin Column 1 x 30 units 1 x 15 units
    Klenow Fragment (3’-5’ exo-) 1 x 30µl 1 x 15µl
    Modification Buffer 1 x 6ml 1 x 3ml
    Modification Powder 1 x 4 vials 1 x 2 vials
    MQ Binding Beads 1 x 3.6ml 1 x 1.8ml
    Primer I (10 μM) 1 x 30µl 1 x 15µl
    Primer U (10 μM) 1 x 30µl 1 x 15µl
    T4 DNA Ligase 1 x 30µl 1 x 15µl
  • Research areas

Associated products

Images

  • Size distribution of library fragments. Post-bisulfite DNA library was prepared from 10 ng of input DNA using ab185907.

Protocols

References

ab185907 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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