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    bisulfite-seq-high-sensitivity-kit-for-illumina-ab185907.pdf

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Kits/ Lysates/ Other Kits Epigenetic kits Histone methylation quantification
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Bisulfite-Seq High Sensitivity Kit (For Illumina®) (ab185907)

  • Datasheet
  • SDS
  • Protocol Booklet
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Size distribution of library fragments

    Key features and details

    • Assay time: 8 hr
    • Sensitivity: 0.5 ng

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    Overview

    • Product name

      Bisulfite-Seq High Sensitivity Kit (For Illumina®)
    • Sensitivity

      < 0.5 ng
    • Assay time

      8h 00m
    • Product overview

      ab185907 is designed to carry out bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit. The DNA library is constructed directly from bisulfite-converted DNA generated from a small amount of input DNA (500 pg to 500 ng). Intended applications include whole genome bisulfite sequencing, oxidative bisulfite sequencing, reduced representative bisulfite sequencing, and various other bisulfite-next generation sequencing techniques. The optimized protocol and components of the kit allow the DNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using sub-nanogram quantities of bisulfite converted DNA.

       

    • Notes

      DNA methylation occurs by the covalent addition of a methyl group (CH3) at the 5-carbon of the cytosine ring, resulting in 5-methylcytosine (5-mC). DNA methylation is essential in regulating gene expression in nearly all biological processes including development, growth, and differentiation. Alterations in DNA methylation have been demonstrated to cause a change in gene expression. Genome-wide analysis of DNA methylation could provide valuable information for discovering epigenetic markers used for disease diagnosis, and potential targets used for therapeutics. Bisulfite sequencing via next-generation sequencing technologies allow for high volume, lower cost output of DNA sequence data towards a better understanding of DNA methylation.

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 24 tests 12 tests
      10X dA-Tailing Buffer 1 x 80µl 1 x 40µl
      10X End Repair Buffer 1 x 80µl 1 x 40µl
      2X HiFi PCR Master Mix 1 x 320µl 1 x 160µl
      2X Ligation Buffer 1 x 500µl 1 x 250µl
      5X Conversion Buffer 1 x 100µl 1 x 50µl
      Adaptors (50 μM) 1 x 30µl 1 x 15µl
      Conversion Enzyme Mix 1 x 30µl 1 x 15µl
      Conversion Primer 1 x 52µl 1 x 26µl
      Desulphonation Solution 1 x 140µl 1 x 70µl
      DNA Binding Solution 1 x 12ml 1 x 6ml
      Elution Buffer 1 x 2ml 1 x 1ml
      Elution Solution 1 x 1ml 1 x 0.5ml
      End Repair Enzyme Mix 1 x 50µl 1 x 25µl
      F-Collection Tube 1 x 30 units 1 x 15 units
      F-Spin Column 1 x 30 units 1 x 15 units
      Klenow Fragment (3’-5’ exo-) 1 x 30µl 1 x 15µl
      Modification Buffer 1 x 6ml 1 x 3ml
      Modification Powder 1 x 4 vials 1 x 2 vials
      MQ Binding Beads 1 x 3.6ml 1 x 1.8ml
      Primer I (10 μM) 1 x 30µl 1 x 15µl
      Primer U (10 μM) 1 x 30µl 1 x 15µl
      T4 DNA Ligase 1 x 30µl 1 x 15µl
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Histone methylation quantification

    Associated products

    • Related Products

      • Genomic DNA Isolation Kit (ab65358)

    Images

    • Size distribution of library fragments
      Size distribution of library fragments

      Size distribution of library fragments. Post-bisulfite DNA library was prepared from 10 ng of input DNA using ab185907.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab185907? Please let us know so that we can cite the reference in this datasheet.

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