Key features and details
- Rabbit polyclonal to BIT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BIT1 antibody
See all BIT1 primary antibodies
DescriptionRabbit polyclonal to BIT1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human BIT1 aa 129-179. The exact sequence is proprietary.
Database link: Q9Y3E5
- WB: HeLa, HEK-293T and Jurkat cell lysate.
This product was previously labelled as PTRH2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 19 kDa.|
FunctionThe natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
Sequence similaritiesBelongs to the PTH2 family.
- Information by UniProt
- Bcl 2 inhibitor of transcription 1 antibody
- Bcl-2 inhibitor of transcription 1 antibody
- BIT 1 antibody
All lanes : Anti-BIT1 antibody (ab226472) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 3 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226472 has not yet been referenced specifically in any publications.