Key features and details
- Rabbit polyclonal to BIT1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-BIT1 antibody
See all BIT1 primary antibodies
DescriptionRabbit polyclonal to BIT1
This antibody is specific for BIT1.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Human BIT1 (N terminal).
(Peptide available as
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). Can be blocked with PTRH2 peptide (ab39732).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
Sequence similaritiesBelongs to the PTH2 family.
- Information by UniProt
- Bcl 2 inhibitor of transcription 1 antibody
- Bcl-2 inhibitor of transcription 1 antibody
- BIT 1 antibody
Lane 1 : Anti-BIT1 antibody (ab36990) at 1 µg/ml
Lane 2 : Anti-BIT1 antibody (ab36990) at 2 µg/ml
Lane 3 : Anti-BIT1 antibody (ab36990) at 4 µg/ml
All lanes : A20 cell lysate.
Lysates/proteins at 15 µg per lane.
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
The arrow next to the image points to the BIT1 band.
ab36990 at 2µg/ml staining BIT1 in 3T3 cells by ICC/IF
Immunofluorescence of Bit1 in 3T3 cells using ab36990 at 20 ug/ml.
ab36990 has been referenced in 2 publications.
- Liu Y et al. Bit1-a novel regulator of astrocyte function during retinal development: proliferation, migration, and paracrine effects on vascular endothelial cell. Hum Cell 32:418-427 (2019). PubMed: 31368047
- Duthie KA et al. Proteomics analysis of interleukin (IL)-7-induced signaling effectors shows selective changes in IL-7Ralpha449F knock-in T cell progenitors. Mol Cell Proteomics 6:1700-10 (2007). WB ; Mouse . PubMed: 17602185