Overview

  • Product name

  • Description

    Rabbit polyclonal to BIT1
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for BIT1.

  • Tested applications

    Suitable for: WB, IHC (PFA fixed), ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human BIT1 (N terminal).
    (Peptide available as ab39732)

  • Positive control

    • A20 cell lysate.
  • General notes

     This product was previously labelled as PTRH2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab36990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC (PFA fixed)
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 2 µg/ml.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). Can be blocked with PTRH2 peptide (ab39732).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
      Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
    • Sequence similarities

      Belongs to the PTH2 family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • Bcl 2 inhibitor of transcription 1 antibody
      • Bcl-2 inhibitor of transcription 1 antibody
      • BIT 1 antibody
      • BIT1 antibody
      • CGI 147 antibody
      • CGI147 antibody
      • mitochondrial antibody
      • Peptidyl tRNA hydrolase 2 mitochondrial antibody
      • Peptidyl-tRNA hydrolase 2 antibody
      • PTH 2 antibody
      • PTH2 antibody
      • PTH2_HUMAN antibody
      • PTRH 2 antibody
      • PTRH2 antibody
      see all

    Images

    • Lane 1 : Anti-BIT1 antibody (ab36990) at 1 µg/ml
      Lane 2 : Anti-BIT1 antibody (ab36990) at 2 µg/ml
      Lane 3 : Anti-BIT1 antibody (ab36990) at 4 µg/ml

      All lanes : A20 cell lysate.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/10000 dilution

      Predicted band size: 20 kDa
      Observed band size: 23 kDa
      why is the actual band size different from the predicted?



      The arrow next to the image points to the BIT1 band.

    • ab36990 at 2µg/ml staining BIT1 in 3T3 cells by ICC/IF

    • Immunofluorescence of Bit1 in 3T3 cells using ab36990 at 20 ug/ml.

    References

    This product has been referenced in:

    • Liu Y  et al. Bit1-a novel regulator of astrocyte function during retinal development: proliferation, migration, and paracrine effects on vascular endothelial cell. Hum Cell 32:418-427 (2019). Read more (PubMed: 31368047) »
    • Duthie KA  et al. Proteomics analysis of interleukin (IL)-7-induced signaling effectors shows selective changes in IL-7Ralpha449F knock-in T cell progenitors. Mol Cell Proteomics 6:1700-10 (2007). WB ; Mouse . Read more (PubMed: 17602185) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab36990.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up