Overview

  • Product name

  • Description

    Rabbit polyclonal to BIT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BIT1 (internal sequence).
    (Peptide available as ab176553)

  • Positive control

    • Daudi cell lysate. Human small intestine tissue.
  • General notes

     This product was previously labelled as PTRH2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab62554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
    Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
  • Sequence similarities

    Belongs to the PTH2 family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bcl 2 inhibitor of transcription 1 antibody
    • Bcl-2 inhibitor of transcription 1 antibody
    • BIT 1 antibody
    • BIT1 antibody
    • CGI 147 antibody
    • CGI147 antibody
    • mitochondrial antibody
    • Peptidyl tRNA hydrolase 2 mitochondrial antibody
    • Peptidyl-tRNA hydrolase 2 antibody
    • PTH 2 antibody
    • PTH2 antibody
    • PTH2_HUMAN antibody
    • PTRH 2 antibody
    • PTRH2 antibody
    see all

Images

  • Lane 1 : Anti-BIT1 antibody (ab62554) at 1 µg/ml
    Lane 2 : Anti-BIT1 antibody (ab62554) at 2 µg/ml
    Lane 3 : Anti-BIT1 antibody (ab62554) at 4 µg/ml

    All lanes : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • ab62554, at 10µg/ml, staining BIT1 in human small intestine by immunohistochemistry using paraffin-embedded tissue.

  • Immunofluorescence of Bit1 in Human Small Intestine cells using ab62554 at 20 ug/ml.

References

ab62554 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.
I can confirm that the exact immunogen sequence is proprietary, but it is from within aa51-100 of NP_057161.
I hope this information is helpful and wish you good luck with your research project.

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