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Cell Biology Apoptosis Mitochondrial
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RecombinantRabMAb

Recombinant Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)
  • Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5165] to BIT1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

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Protein
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Recombinant Human BIT1 protein (ab101645)
Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

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Overview

  • Product name

    Anti-BIT1 antibody [EPR5165] - BSA and Azide free
    See all BIT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5165] to BIT1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BIT1 (C terminal). The exact sequence is proprietary.

  • General notes

    ab247820 is the carrier-free version of ab109291 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247820 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as PTRH2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5165
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-BIT1 antibody [EPR5165] (ab109291)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human BIT1 protein (ab101645)

Applications

Our Abpromise guarantee covers the use of ab247820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 19,21 kDa (predicted molecular weight: 19 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
      Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
    • Sequence similarities

      Belongs to the PTH2 family.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q9Y3E5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51651 Human
      • Omim: 608625 Human
      • SwissProt: Q9Y3E5 Human
      • Unigene: 12677 Human
      • Alternative names

        • Bcl 2 inhibitor of transcription 1 antibody
        • Bcl-2 inhibitor of transcription 1 antibody
        • BIT 1 antibody
        • BIT1 antibody
        • CGI 147 antibody
        • CGI147 antibody
        • mitochondrial antibody
        • Peptidyl tRNA hydrolase 2 mitochondrial antibody
        • Peptidyl-tRNA hydrolase 2 antibody
        • PTH 2 antibody
        • PTH2 antibody
        • PTH2_HUMAN antibody
        • PTRH 2 antibody
        • PTRH2 antibody
        see all

      Images

      • Western blot - Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)
        Western blot - Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)
        All lanes : Anti-BIT1 antibody [EPR5165] (ab109291) at 1/10000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Raji cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 19 kDa



        This data was developed using ab109291, the same antibody clone in a different buffer formulation.

         

      • Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)
        Anti-BIT1 antibody [EPR5165] - BSA and Azide free (ab247820)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247820? Please let us know so that we can cite the reference in this datasheet.

      ab247820 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab247820.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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