Recombinant Anti-BIVM antibody [EPR9109] (ab137113)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-BIVM antibody [EPR9109]
See all BIVM primary antibodies -
Description
Rabbit monoclonal [EPR9109] to BIVM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human BIVM aa 450-550 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, Jurkat and Raji cell lysates; breast carcinoma and kidney tissues.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9109 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab137113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. It is recommended to use an HRP/AP polymerized secondary antibody. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
BIVM (for basic, immunoglobulin like variable motif containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503 amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 54841 Human
- Entrez Gene: 246229 Mouse
- SwissProt: Q86UB2 Human
- SwissProt: Q8CBX9 Mouse
- Unigene: 288809 Human
- Unigene: 386883 Mouse
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Alternative names
- AI854267 antibody
- Basic immunoglobulin like variable motif containing antibody
- FLJ44301 antibody
see all
Images
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![Western blot - Anti-BIVM antibody [EPR9109] (ab137113)](https://www.abcam.com/ps/products/137/ab137113/Images/BIVM-Primary-antibodies-ab137113-1.jpg)
Western blot - Anti-BIVM antibody [EPR9109] (ab137113)All lanes : Anti-BIVM antibody [EPR9109] (ab137113) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIVM antibody [EPR9109] (ab137113)](https://www.abcam.com/ps/products/137/ab137113/Images/BIVM-Primary-antibodies-ab137113-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIVM antibody [EPR9109] (ab137113)Immunohistochemical analysis of paraffin-embedded, formalin-fixed breast carcinoma tissue, staining BIVM using ab137113 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIVM antibody [EPR9109] (ab137113)](https://www.abcam.com/ps/products/137/ab137113/Images/BIVM-Primary-antibodies-ab137113-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIVM antibody [EPR9109] (ab137113)Immunohistochemical analysis of paraffin-embedded, formalin-fixed kidney tissue, staining BIVM using ab137113 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
References
ab137113 has not yet been referenced specifically in any publications.
