Anti-BLAP75 antibody (ab70525)
Key features and details
- Rabbit polyclonal to BLAP75
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BLAP75 antibody -
Description
Rabbit polyclonal to BLAP75 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
A region between residues 275 and 300 of human BLAP75 (NP_079221.1)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
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IP |
Use at 1-4 µg/mg of lysate.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
Target
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Function
Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability. -
Sequence similarities
Belongs to the RMI1 family. -
Cellular localization
Nucleus. Forms foci in response to DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 80010 Human
- Omim: 610404 Human
- SwissProt: Q9H9A7 Human
- Unigene: 726327 Human
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Alternative names
- BLAP75 antibody
- BLM associated polypeptide 75 kDa antibody
- BLM associated protein 75 kDa antibody
see all
Images
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All lanes : Anti-BLAP75 antibody (ab70525) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 170 kDa, 190 kDa, 238 kDa, 32 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB.
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Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab70525 has been referenced in 3 publications.
- González-Acosta D et al. PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. EMBO J 40:e106355 (2021). PubMed: 34128550
- Jia Y et al. Relationship between blood lactic acid, blood procalcitonin, C-reactive protein and neonatal sepsis and corresponding prognostic significance in sick children. Exp Ther Med 14:2189-2193 (2017). PubMed: 28962140
- Ke Y et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB ; Human . PubMed: 21743438