Product nameAnti-BLAP75 antibody
See all BLAP75 primary antibodies
DescriptionRabbit polyclonal to BLAP75
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A region between residues 275 and 300 of human BLAP75 (NP_079221.1)
- Whole cell lysate from HeLa cells. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionEssential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
Sequence similaritiesBelongs to the RMI1 family.
Cellular localizationNucleus. Forms foci in response to DNA damage.
- Information by UniProt
- BLAP75 antibody
- BLM associated polypeptide 75 kDa antibody
- BLM associated protein 75 kDa antibody
All lanes : Anti-BLAP75 antibody (ab70525) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 170 kDa, 190 kDa, 238 kDa, 32 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB.
ICC/IF image of ab70525 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70525 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.
This product has been referenced in:
- Jia Y et al. Relationship between blood lactic acid, blood procalcitonin, C-reactive protein and neonatal sepsis and corresponding prognostic significance in sick children. Exp Ther Med 14:2189-2193 (2017). Read more (PubMed: 28962140) »
- Ke Y et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB ; Human . Read more (PubMed: 21743438) »