Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12157(B)] to BLAP75
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-BLAP75 antibody [EPR12157(B)]
See all BLAP75 primary antibodies
DescriptionRabbit monoclonal [EPR12157(B)] to BLAP75
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BLAP75 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H9A7
- HT-1080, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 70 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionEssential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
Sequence similaritiesBelongs to the RMI1 family.
Cellular localizationNucleus. Forms foci in response to DNA damage.
- Information by UniProt
- BLAP75 antibody
- BLM associated polypeptide 75 kDa antibody
- BLM associated protein 75 kDa antibody
Immunofluorescent analysis of HeLa cells labeling BLAP75 with ab172625 at 1/50 dilution.
All lanes : Anti-BLAP75 antibody [EPR12157(B)] (ab172625) at 1/1000 dilution
Lane 1 : HT-1080 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
ab172625 has not yet been referenced specifically in any publications.