Overview

  • Product name

    Anti-BLAP75 antibody [EPR12157(B)]
    See all BLAP75 primary antibodies
  • Description

    Rabbit monoclonal [EPR12157(B)] to BLAP75
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BLAP75 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H9A7

  • Positive control

    • HT-1080, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12157(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
    • Sequence similarities

      Belongs to the RMI1 family.
    • Cellular localization

      Nucleus. Forms foci in response to DNA damage.
    • Information by UniProt
    • Database links

    • Alternative names

      • BLAP75 antibody
      • BLM associated polypeptide 75 kDa antibody
      • BLM associated protein 75 kDa antibody
      • BLM-associated protein of 75 kDa antibody
      • C9orf76 antibody
      • FAAP75 antibody
      • FLJ12888 antibody
      • Homolog of yeast RecQ mediated genome instability 1 antibody
      • RecQ mediated genome instability 1 antibody
      • RecQ-mediated genome instability protein 1 antibody
      • Rmi1 antibody
      • RMI1 homolog antibody
      • RMI1 RecQ mediated genome instability 1 homolog antibody
      • RMI1_HUMAN antibody
      • RP11-346I8 antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling BLAP75 with ab172625 at 1/50 dilution.

    • All lanes : Anti-BLAP75 antibody [EPR12157(B)] (ab172625) at 1/1000 dilution

      Lane 1 : HT-1080 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    References

    ab172625 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172625.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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