Anti-BLBP antibody (ab110099)
Key features and details
- Goat polyclonal to BLBP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BLBP antibody
See all BLBP primary antibodies -
Description
Goat polyclonal to BLBP -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig -
Immunogen
Synthetic peptide corresponding to Human BLBP (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- Human cerebellum, heart or skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. -
Tissue specificity
Expressed in brain and other neural tissues. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 777787 Cow
- Entrez Gene: 2173 Human
- Omim: 602965 Human
- SwissProt: Q09139 Cow
- SwissProt: O15540 Human
- Unigene: 26770 Human
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Alternative names
- B FABP antibody
- B-FABP antibody
- BFABP antibody
see all
Images
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ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human skeletal muscle tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab110099 staining BLBP in Xenopus laevis gindbrain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution, permeabilized with 0.4% Triton X-100 in 3% normal horse serum and blocked with 3% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were incubated with primary antibody (1/100 in 0.4% Triton X-100 in 3% normal horse serum) for 12 hours at 4°C. An undiluted HRP-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.
Datasheets and documents
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Datasheet download
References (2)
ab110099 has been referenced in 2 publications.
- Van Houcke J et al. Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri. Aging Cell 20:e13464 (2021). PubMed: 34428340
- Nascimento JM et al. Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities. Front Cell Dev Biol 7:303 (2019). PubMed: 31850342